Alcohol dehydrogenase in human tissues: Localisation of transcripts coding for five classes of the enzyme
Alcohol dehydrogenase in human tissues: Localisation of transcripts coding for five classes of the enzyme
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DOI：
10.1016/s0014-5793(96)01204-5
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发表时间：
1996-11-18
期刊：
影响因子：
3.5
通讯作者：
Hoog, JO
中科院分区：
文献类型：
--
作者：
Estonius, M;Svensson, S;Hoog, JO
通过评估23个成人酸四胎儿组织中的mRNA水平，研究了已鉴定的五类人类乙醇脱氢酶的组织分布，在大多数组织中发现了I类乙醇脱氢酶，不包括脑和胎盘，但组织之间的表达水平差异很大，III类转录本的分布模式与管家酶的分布模式一致，而相比之下，IV类转录本仅在胃中发现，大多数类别检测到多种长度的转录本，这是由于不同的基因产物产生的使用不同的多聚腺苷酸信号或来自不同基因位点的转录，II类和V类都显示出肝脏富集表达的模式，然而，在II类的胃、胰腺和小肠中以及V类的胎儿肾和小肠中也检测到低mRNA水平。与成人肝脏中的水平相比，胎儿肝脏中存在V类转录物的水平显着较高，这表明人类V类主要是胎儿乙醇脱氢酶。
Tissue distribution of the five identified classes of human alcohol dehydrogenase was studied by assessment of mRNA levels in 23 adult acid four fetal tissues, Alcohol dehydrogenase of class I was found in most tissues, brain and placenta excluded, but expression levels among tissues differed widely, The distribution pattern of class III transcripts was consistent with those of housekeeping enzymes,while, in contrast, class IV transcripts were found only in stomach, Transcripts of multiple length were detected for most classes and were due to different gene products arising through the use of different poly-A signals or transcription from different gene loci, Both class II and class V showed a pattern of liver-enriched expression, However, low mRNA levels were detected also in stomach, pancreas and small intestine for class II, and in fetal kidney and small intestine for class V, Significantly higher levels of class V transcripts were present in fetal liver when compared with levels in adult liver, which suggests that human class V is a predominantly fetal alcohol dehydrogenase.