VIRAL HEMAGGLUTININ AUGMENTS PEPTIDE-SPECIFIC CYTOTOXIC T-CELL RESPONSES
VIRAL HEMAGGLUTININ AUGMENTS PEPTIDE-SPECIFIC CYTOTOXIC T-CELL RESPONSES
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DOI：
10.1002/eji.1830231032
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发表时间：
1993-10-01
影响因子：
5.4
通讯作者：
VONHOEGEN, P
中科院分区：
文献类型：
--
作者：
ERTEL, C;MILLAR, NS;VONHOEGEN, P
为了增加对肽特异性细胞溶解T细胞（CTL）的诱导，我们研究了新城疫病毒（NDV）血凝素-神经氨酸酶（HN）基因产物对肽特异性CTL活化的影响。在体外(i)用肽脉冲型同源成纤维细胞（Ltk-）作为抗原提呈细胞再刺激抗流感核蛋白肽50-63 （NP 50-63）免疫的CH3小鼠脾脏细胞，同时（ii）感染新冠病毒或（iii）稳定转染新冠病毒的HN cDNA。在用肽脉冲Ltk细胞再刺激的培养中，由于感染或转染，共表达病毒血凝素，观察到肽特异性CTL反应增加了六倍以上。当用经过NDV感染修饰的抗原呈递细胞刺激次优培养物时，对其他类型抗原（主要组织相容性复合物异体抗原，肿瘤抗原的次要组织相容性抗原）的CTL反应也出现了类似的增强。这些发现提示NDV或病毒HN在细胞或肿瘤细胞抗原上表达可发挥T细胞共刺激功能。
In attempt to increase the induction of peptide-specific cytolytic T cells (CTL) we investigated the effect of the Newcastle disease virus (NDV) hemagglutinin-neuraminidase (HN) gene product on the activation of peptide-specific CTL. Spleen cells of CH3 mice immunized against the influenza nucleoprotein peptide 50-63 (NP 50-63) were restimulated in vitro (i) with peptide-pulsed syngeneic fibroblast cells (Ltk-) as antigen-presenting cells, which were in addition (ii) infected with NDV or (iii) stably transfected with the HN cDNA of NDV. A greater than sixfold increase in peptide-specific CTL responses was observed in cultures restimulated with peptide-pulsed Ltk- cells which coexpressed viral hemagglutinin due to either infection or transfection.A similar augmentation was seen in CTL responses against other types of antigen (major histocompatibility complex alloantigens, minor histocompatibility antigens of tumor antigens) when suboptimal cultures were stimulated with the respective antigen-presenting cells modified by NDV infection. These findings suggest that NDV or viral HN expressed on antigen-present in cells or tumor cells can exert a T cell co-stimulatory function.