Separate lineages of T cells expressing the αβ and γδ receptors
Separate lineages of T cells expressing the αβ and γδ receptors
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表达 αβ 和 γδ 受体的 T 细胞的不同谱系
DOI：
10.1038/338430a0
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发表时间：
1989
期刊：
影响因子：
64.8
通讯作者：
D. Baltimore
中科院分区：
文献类型：
--
作者：
A. Winoto;D. Baltimore
T细胞抗原受体是由αβ或γδ链组成的异源二聚体分子。αβ受体分子主要在CD 4 + CD 8 −和CD 4 − CD 8 + T细胞（分别为辅助T细胞和杀伤T细胞）中表达，而γδ受体分子主要在CD 4 − CD 8- T细胞中表达1 -3。在胸腺发育过程中，CD 4 + CD 8 −和CD 4 − CD 8 + T细胞来源于一类CD 4 − CD 8 − T细胞4，这就提出了一个问题：细胞重排其γδ受体后是否通过受体基因的进一步重排产生αβ T细胞，或者受体基因的重排和表达是否发生在不同的谱系中。δ -链基因位于Vα（可变）和Jα（连接）基因片段之间5，6，当允许α-和β-受体发生重排时，这些片段之间的DNA作为小环缺失，可以从发育中的胸腺细胞中分离7，8。因此，可以研究α环中δ链基因的重排状态，以了解α链和δ链的表达是在平行谱系中发生还是在谱系中顺序发生。我们发现T细胞受体α环中的δ链基因具有种系构型，表明αβ和γδ T细胞是不同的谱系。
THE T-cell antigen receptor is a heterodimer molecule composed of either αβ or γδ chains. The αβ receptor molecules are expressed mainly in CD4+ CD8− and CD4−CD8+ T cells (helper and killer T cells respectively), whereas the γδ receptor molecules are expressed mainly in CD4−CD8- T cells1–3. CD4+CD8− and CD4−CD8+ T cells arise from a class of CD4−CD8− T cells during thymus development4, raising the question of whether cells rearranging their γδ receptors later give rise to αβ T cells by further rearrangements of their receptor genes, or whether rearrangements and expression of the receptor genes occur in separate lineages. The δ -chain gene is located between the Vα (variable) and Jα(joining) gene segments5,6 and when the rearrangements allowing α- and β-receptors occur, the DNA between these segments is deleted as small circles which can be isolated from developing thymocytes7,8. The rearrangement status of the δ -chain gene in the α-circles can therefore be investigated to see whether α-chain and δ -chain expression occur in parallel lineages or sequentially within a lineage. We find that the δ -chain gene in the T-cell receptor α-circles has a germline configuration, indicating that αβ and γδ T cells are distinct lineages.