Structures of oligosaccharides derived from Cladosiphon okamuranus fucoidan by digestion with marine bacterial enzymes
Structures of oligosaccharides derived from Cladosiphon okamuranus fucoidan by digestion with marine bacterial enzymes
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DOI：
10.1007/s10126-002-0107-9
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发表时间：
2003-11-01
影响因子：
3
通讯作者：
Kato, I
中科院分区：
文献类型：
--
作者：
Sakai, T;Ishizuka, K;Kato, I
利用岩藻聚糖的海洋细菌Fucophilus fucoidanolyticus在含有岩藻聚糖的培养基中培养。梭利用F.通过核磁共振分析确定了它们的结构。它们的一些结构由一个结构通式表示，（-3L-Fucpalpha 1 - 3L-Fucp（4-O-硫酸酯）α 1 -3L-Fucp（4-O-硫酸酯）α 1 -3（D-GlcpUA α 1 -2）L-Fucpalpha 1）-3L-Fucp（4-O-硫酸酯）α 1 - 3L-Fucp（4-O-硫酸酯）α 1 - 3L-Fucp（m = 0、1、2或3）。我们的结论是，所获得的所有寡糖都来自于一种含硫酸化岩藻糖的C. Okamuranus，其具有重复单元（-3L-Fucpalpha 1 - 3L-Fucp（4-O-硫酸盐）alpha 1 - 3L-Fucp（4-O-硫酸盐）alpha 1 -3（D-GlcpUA alpha 1 -2）L-Fucpalpha 1-）。
A fucoidan-utilizing marine bacterium, Fucophilus fucoidanolyticus, was cultivated in medium containing fucoidan from Cladosiphon okamuranus. The C. okamuranus fucoidan was digested into oligosaccharides with the intracellular enzymes of F. fucoidanolyticus, and their structures were determined by nuclear magnetic resonance analyses. Some of their structures are represented by one general structural formula, (-3L-Fucpalpha1-3L-Fucp(4-O-sulfate)alpha1-3L-Fucp(4-O-sulfate)alpha1-3(D-GlcpUAalpha1-2)L-Fucpalpha1)-3L-Fucpalpha1-3L-Fucp(4-O-sulfate)alpha1-3L-Fucp(4-O-sulfate) alpha1-3L-Fucp (m = 0, 1, 2, or 3). We concluded that all oligosaccharides obtained were derived from a sulfated-fucose-containing polysaccharide of C. okamuranus, which has a repeating unit of (-3L-Fucpalpha1-3L-Fucp(4-O-sulfate)alpha1-3L-Fucp(4-O-sulfate)alpha1-3 (D-GlcpUAalpha1-2) L-Fucpalpha1-).