Mitochondrial gene expression is regulated at multiple levels and differentially in the heart and liver by thyroid hormones
Mitochondrial gene expression is regulated at multiple levels and differentially in the heart and liver by thyroid hormones
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DOI：
10.1007/s00294-008-0194-x
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发表时间：
2008-07-01
期刊：
影响因子：
2.5
通讯作者：
Fernandez-Silva, Patricio
中科院分区：
文献类型：
--
作者：
Fernandez-Vizarra, Erika;Enriquez, Jose A.;Fernandez-Silva, Patricio
氧化磷酸化系统（OXPHOS）的生物发生需要细胞核和线粒体基因组的协调表达。甲状腺激素在细胞生长和分化中起重要作用，并且是线粒体生物合成的主要效应物之一。为了确定线粒体DNA的表达是如何调节的，我们研究了两种不同的组织，心脏和肝脏，在体内和体外的甲状腺激素状态的反应。我们在这里表明，mtDNA的表达是一个严格调控的过程，几个层次的控制可以同时发生。此外，我们发现，在两种组织之间的mtDNA表达和它们的相关性的控制机制不同，基因剂量重要的心脏，而转录速率和翻译效率有更多的重量在肝细胞。另一个有趣的区别是甲状腺激素对心脏线粒体转录缺乏直接影响。
Biogenesis of the oxidative phosphorylation system (OXPHOS) requires the coordinated expression of the nuclear and the mitochondrial genomes. Thyroid hormones play an important role in cell growth and differentiation and are one of the main effectors in mitochondrial biogenesis. To determine how mtDNA expression is regulated, we have investigated the response of two different tissues, the heart and liver, to the thyroid hormone status in vivo and in vitro. We show here that mtDNA expression is a tightly regulated process and that several levels of control can take place simultaneously. In addition, we show that the mechanisms operating in the control of mtDNA expression and their relevance differ between the two tissues, being gene dosage important only in heart while transcription rate and translation efficiency have more weight in liver cells. Another interesting difference is the lack of a direct effect of thyroid hormones on heart mitochondrial transcription.