Analysis of genetic diversity and population structure of confectionery sunflower (Helianthus annuus L.) native to Iran.
Analysis of genetic diversity and population structure of confectionery sunflower (Helianthus annuus L.) native to Iran.
复制标题
DOI：
10.1007/s12892-015-0052-6
复制
发表时间：
2016-01-01
影响因子：
--
通讯作者：
Hatamnia, A. A.
中科院分区：
文献类型：
--
作者：
Jannatdoust, M.;Darvishzadeh, R.;Hatamnia, A. A.
50个糖果向日葵（Helianthus annuus L.）种群内和种群间的遗传多样性从伊朗不同的地理区域收集的，通过使用微卫星和反转录转座子标记进行评估。SSR位点的等位基因数（Na）在2 ~ 3个之间，平均为2.1个。反转录转座子标记的多态性条带从CR-UR 1位点的7条到CR-816位点的15条，平均值为11.33。利用DARwin 5软件中的邻点连接法对50*5=250个个体进行了等级聚类，将其分为3个类群。利用Structure软件包中的Bayesian方法，将被研究的个体再分为两个亚群体。主坐标分析表明，前两个成分分别解释总方差的7.86%和6.16%。分子方差分析表明，群体内的遗传变异（70%）高于群体间的遗传变异（30%）。居群内个体间的高分子变异可能是由于向日葵植物的高异花受精性质。种群间遗传变异低，可以认为是该地区长期种植糖果向日葵以及区域间种子交换的遗传平衡的结果。传统的假设，即选择不同地理来源的基因型将最大限度地提高育种项目的多样性，并不适用于糖果向日葵。
Genetic diversity within and among 50 populations of confectionery sunflower (Helianthus annuus L.) collected from different geographical areas of Iran was evaluated by using microsatellite and retrotransposon markers. The number of alleles (Na) in SSR loci ranged from 2 to 3 with an average of 2.1. The polymorphic bands in retrotransposon markers ranged from 7 in locus CR-UR1 to 15 in locus CR-816 with a mean value of 11.33. Herarchical clustering of individuals (50*5=250) by neighbor joining method in DARwin5 software subdivided them into three groups. Using Bayesian method in the software pakage of Structure, the studied individuals were subdivided into two sub-populations. Principal coordinate analysis revealed that the two first components explained 7.86 and 6.16% of the total variance, respectively. Analysis of molecular variance revealed a higher level of genetic variation within (70%) than between (30%) populations. High molecular variation among individuals within population possibly is due to high allogamy nature of the sunflower plant. Low genetic variation observed between populations could be considered as a consequence of genetic equilibrium that has occurred over the long period of cultivation of confectionery sunflower in this area as well as seed exchange among regions. The traditional assumption that selecting genotypes of different geographical origin will maximize the diversity available to a breeding project does not hold in confectionery sunflower.