Expression of fascin-1, an actin-bundling protein, in migrating hepatoblasts during rat liver development
Expression of fascin-1, an actin-bundling protein, in migrating hepatoblasts during rat liver development
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DOI：
10.1007/s00441-008-0683-8
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发表时间：
2008-11-01
影响因子：
3.6
通讯作者：
Lee, Gang-Hong
中科院分区：
文献类型：
--
作者：
Hayashi, Yoshihiro;Toda, Katsumi;Lee, Gang-Hong
Fascin-1 是一种肌动蛋白成束蛋白，位于迁移细胞中微绒毛投射和丝状伪足延伸内的核心肌动蛋白束处。它在正常上皮细胞中低水平表达，但在肿瘤细胞中高水平表达，表明其在肿瘤细胞的侵袭和运动中的重要性。此外，Fascin-1 在人类和小鼠胚胎中表达，尤其是在发育中的神经组织中表达水平很高。在本研究中，我们通过免疫组织化学方法研究了大鼠肝发生早期阶段的fascin-1表达模式。在胚胎日（ED）10.5、ED11.5和ED12.5的肝芽和成肝细胞中检测到Fascin-1的高表达。表达在 ED13.5 时下降，在 ED14.5 时未检测到。这些观察结果表明，Fascin-1 的表达与大鼠肝脏发育早期阶段成肝细胞的迁移活性相关。
Fascin-1 is an actin-bundling protein localized at the core actin bundles within microvillar projections and filopodial extensions in migrating cells. It is expressed at a low level in normal epithelial cells, but at a high level in tumor cells, indicating its importance in the invasion and motility of tumor cells. In addition, fascin-1 is expressed in human and murine embryos, occurring at high levels especially in developing nervous tissues. In this study, we have investigated the expression patterns of fascin-1 immunohistochemically during the early stages of rat hepatogenesis. A high expression of fascin-1 was detected in the liver bud and hepatoblasts at embryonic day (ED) 10.5, ED11.5, and ED12.5. Expression fell by ED13.5 and was not detectable at ED14.5. These observations demonstrate that the expression of fascin-1 is correlated with the migration activity of hepatoblasts during the early stages of liver development in rats.