Aligned electrospun siloxane-doped vaterite/poly (l-lactide) composite fibremats : evaluation of their tensile strength and cell compatibility
Aligned electrospun siloxane-doped vaterite/poly (l-lactide) composite fibremats : evaluation of their tensile strength and cell compatibility
复制标题
定向静电纺丝硅氧烷掺杂球霰石/聚（L-丙交酯）复合纤维材料：评估其拉伸强度和细胞相容性
DOI：
10.1080/09205063.2013.825752
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Toshihiro Kasuga
中科院分区：
文献类型：
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作者：
Noora-Maria Tujunen;Kie Fujikura;Akiko Obata;Toshihiro Kasuga
本课题组研制了硅氧烷掺杂球状沸石/聚L-丙交酯杂化复合材料作为骨修复材料，并成功地制成了无纺布电纺纤维毡。本工作的目的是制备不同SIV含量的电纺SiPVH纤维，并使用小鼠成骨细胞比较其拉伸性能和细胞相容性。结果表明，最大应力随SiV含量的变化呈现出一定的非线性趋势：当SiV含量超过30 wt.%时，最大应力达到最大值，大于40 wt.%SiV时，最大应力显著降低。对取向和随机电纺的SiPVH纤维进行了细胞形态和增殖的检测。细胞接种在排列好的纤维垫上后仅3 h就开始定向，并继续沿着纤维伸长。在随机纤维和定向纤维上培养7天的细胞数量具有可比性，因此定向对细胞的增殖没有负面影响。
Siloxane-doped vaterite (SiV)/poly(l-lactide) hybrid-composite (SiPVH) has been developed in our group as the bone repair material and successfully fabricated into a non-woven electrospun fibremat. The aim of this work is to prepare aligned electrospun SiPVH fibremats with varied SiV content and compare their tensile properties and cell compatibilities using mouse osteoblast-like cells. It was observed that the maximum stress exhibited some non-linear trend as a function of SiV content: the highest stress value was reached with 30 wt.% SiV and decreased significantly with more than 40 wt.% SiV. Cellular morphology and proliferation were taken under examination on both aligned and random electrospun SiPVH fibremats. The cells started to orient themselves only 3 h after seeding on the aligned fibremat and they continued to elongate along the fibres. The number of the cells cultured up to seven days on both random and aligned fibremats was well comparable; therefore the alignment did not show negative effect on the cellular proliferation.