Effect of cell adsorption on photosterilization of Escherichia coli over titanium dioxide-activated charcoal granules

Effect of cell adsorption on photosterilization of Escherichia coli over titanium dioxide-activated charcoal granules
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DOI:
10.1252/jcej.31.922
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发表时间:
1998-12-01
影响因子:
0.8
通讯作者:
Tone, S
Tone, S
中科院分区:
工程技术4区
文献类型:
--
作者:
Horie, Y;Taya, M;Tone, S

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通过溶胶-凝胶法将TiO 2颗粒固定在活性炭颗粒上,用作能够吸附微生物细胞的光灭菌催化剂。在288至303 Ii的温度下测定吸附到TiO 2固定的活性炭(T/AC)颗粒的大肠杆菌细胞的量,并且分别在288和293 K获得最大和最小吸附细胞量。在298 K的温度下,在0.1 - 1.0 kg/m3的T/AC浓度范围内,吸附和悬浮细胞量之间的关系可用Freundlich吸附等温线表示(3),在黑光灯照射下,对大肠杆菌进行了杀菌试验。在T/AC颗粒悬浮液中,温度为288 ~ 303 Ii,T/AC浓度为0 ~ 0.1kg/m ~ 3,对大肠杆菌细胞的杀菌率随温度的变化而变化,最高和最低杀菌率分别在288和293 Ii时达到。在不同灭菌条件下,基于串联事件模型确定了表观灭菌速率常数,通过考虑细胞对T/AC颗粒的吸附,速率常数可以与T/AC颗粒的浓度相关。
TiO2 particles were immobilized on activated charcoal granules by means of a sol-gel method to use as a photosterilization catalyst capable of adsorbing microbial cells. The amounts of Escherichia coli cells adsorbed to the TiO2-immobilized activated charcoal (T/AC) granules were determined at temperatures of 288 to 303 Ii, and the maximum and minimum amounts of adsorbed cells were obtained at 288 and 293 K, respectively, At a temperature of 298 K, the relation between the amounts of adsorbed and suspended cells can be expressed by the Freundlich adsorption isotherm in the range of T/AC concentrations of 0.1 to 1.0 kg/m(3), Under light irradiation with a black light fluorescent lamp, sterilization of E. coli cells was carried out in the slurry of T/AC granules at temperatures of 288 to 303 Ii and T/AC concentrations of 0 to 0.1 kg/m3, The sterilization rates changes depending on the temperatures, and the highest and lowest rates are obtained at 288 and 293 Ii, respectively. The apparent sterilization rate constants are determined on the basis of a series-event model under various sterilization conditions, The rate constants can he correlated with the concentration of T/AC granules by taking into account the adsorption of cells to the T/AC granules.