ZmCCD10a Encodes a Distinct Type of Carotenoid Cleavage Dioxygenase and Enhances Plant Tolerance to Low Phosphate
ZmCCD10a Encodes a Distinct Type of Carotenoid Cleavage Dioxygenase and Enhances Plant Tolerance to Low Phosphate
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ZmCCD10a 编码一种独特类型的类胡萝卜素裂解双加氧酶并增强植物对低磷酸盐的耐受性
DOI：
10.1104/pp.20.00378
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发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Li Xuexian
中科院分区：
文献类型：
--
作者：
Zhong Yanting;Pan Xiaoying;Wang Ruifeng;Xu Jiuliang;Guo Jingyu;Yang Tingxue;Zhao Jianyu;Nadeem Faisal;Liu Xiaoting;Shan Hongyan;Xu Yanjun;Li Xuexian
类胡萝卜素裂解双加氧酶(CCDs)驱动类胡萝卜素分解代谢，产生具有特殊发育、生态和农业意义的类胡萝卜素及其直接衍生物。CCD基因是如何随着物种多样性而进化的，以及由此产生的谷类作物的功能新颖性在很大程度上仍然难以捉摸。我们构建了一个统一的CCDS四分支系统发育树，揭示了一个先前未锚定的基本分支CCD10，CCD10经历了高度动态的复制或丢失事件，即使在草科中也是如此。不同于玉米CCD8和玉米赤霉素酮合成酶的裂解位点，玉米ZmCCD10a在5，6(5‘，6’)和9，10(9‘，10’)位裂解结构不同的类胡萝卜素，在大肠杆菌中产生C8(6-甲基-5-庚烯-2-酮)和C13(香叶内酮、α-紫罗兰酮和β-紫罗兰酮)类胡萝卜素。ZmCCD10a定位于叶绿体中，切割植物中的新黄质、紫黄质、花青素、叶黄素、玉米黄素和β-胡萝卜素，证实了ZmCCD10a和ZAS的功能分化。ZmCCD10a在玉米和特温特(玉米、玉米、华中玉米、华东玉米、玉米和双果玉米)根中的表达受到磷(PI)限制的显著刺激。ZmCCD10在拟南芥中的过表达通过优先向地上部分配磷来提高植物对磷限制的耐受性。因此，ZmCCD10aa编码了一种独特的促进植物对PI限制耐受的Ccd。此外，ZmCCD10a的沉默和过表达导致了磷酸饥饿反应调节因子1(Phr1)和PI转运体表达的一致变化，并通过ZmPHR1；1和ZmPHR1；2顺式调节ZmCCD10a的表达，暗示了一条可能涉及ZmCCD10a的调节途径，调节PI的分配。
Carotenoid cleavage dioxygenases (CCDs) drive carotenoid catabolism to produce various apocarotenoids and immediate derivatives with particular developmental, ecological, and agricultural importance. HowCCDgenes evolved with species diversification and the resulting functional novelties in cereal crops have remained largely elusive. We constructed a unified four-clade phylogenetic tree ofCCDs, revealing a previously unanchored basal cladeCCD10.CCD10underwent highly dynamic duplication or loss events, even in the grass family. Different from cleavage sites of CCD8 and ZAXINONE SYNTHASE (ZAS), maize (Zea mays) ZmCCD10a cleaved differentially structured carotenoids at 5, 6 (5′, 6') and 9, 10 (9', 10') positions, generating C8(6-methyl-5-hepten-2-one) and C13(geranylacetone, α-ionone, and β-ionone) apocarotenoids inEscherichia coli. Localized in plastids, ZmCCD10a cleaved neoxanthin, violaxanthin, antheraxathin, lutein, zeaxanthin, and β-carotene in planta, corroborating functional divergence of ZmCCD10a and ZAS.ZmCCD10aexpression was dramatically stimulated in maize and teosinte (Z. maysssp.parviglumis,Z. maysssp.huehuetenangensis,Zea luxurians, andZea diploperennis) roots by phosphate (Pi) limitation.ZmCCD10asilencing favored phosphorus retention in the root and reduced phosphorus and biomass accumulation in the shoot under low Pi. Overexpression ofZmCCD10ain Arabidopsis (Arabidopsis thaliana) enhanced plant tolerance to Pi limitation by preferential phosphorus allocation to the shoot. Thus,ZmCCD10aencodes a unique CCD facilitating plant tolerance to Pi limitation. Additionally,ZmCCD10asilencing and overexpression led to coherent alterations in expression ofPHOSPHATE STARVATION RESPONSE REGULATOR 1(PHR1) and Pi transporters, and cis-regulation ofZmCCD10aexpression by ZmPHR1;1 and ZmPHR1;2 implies a probableZmCCD10a-involved regulatory pathway that adjusts Pi allocation.