Immunodetectable cyclin D(1)is associated with oestrogen receptor but not Ki67 in normal, cancerous and precancerous breast lesions.
Immunodetectable cyclin D(1)is associated with oestrogen receptor but not Ki67 in normal, cancerous and precancerous breast lesions.
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DOI：
10.1054/bjoc.2001.1705
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发表时间：
2001-04-20
影响因子：
8.8
通讯作者：
Sloane, J P
中科院分区：
文献类型：
--
作者：
Shoker, B S;Jarvis, C;Davies, M P;Iqbal, M;Sibson, D R;Sloane, J P
Cyclin D1与细胞周期调节有关，最近已被证明可刺激雌激素受体（ER）的转录功能。此外，在正常乳腺中，ER与增殖标志物Ki67的表达呈负相关，这表明ER阳性细胞不分裂，或者受体在细胞进入周期时下调。这种重要的关系在许多er阳性癌症和癌前乳腺病变中被打破，因为受体经常在增殖细胞上被检测到。本研究的目的是利用免疫荧光双染色法确定从正常到浸润性乳腺癌的个体细胞内ER、Ki67和细胞周期蛋白d1之间的相互作用。我们发现，在正常乳腺中，ER与细胞周期蛋白d1的表达呈正相关。相反，cyclin d1与Ki67的表达呈负相关。其他癌前病变和癌性乳腺病变也有类似的发现。因此，单个细胞中免疫检测到的细胞周期蛋白d1似乎与良性或恶性乳腺的细胞周期进展无关，但可能与内质网有重要的相互作用。©2001癌症研究运动http://www.bjcancer.com
Cyclin D1 is associated with cell cycle regulation and has more recently been shown to stimulate the transcriptional functions of the oestrogen receptor (ER). Furthermore, in normal breast there is a negative association between expression of ER and the proliferation marker Ki67 indicating that either ER positive cells are non-dividing or that the receptor is down-regulated as cells enter cycle. This important relationship breaks down in many ER-positive cancers and precancerous breast lesions where the receptor is often detected on proliferating cells. The aims of the present study were to determine the interplay between ER, Ki67 and cyclin D 1 in individual cells within the spectrum of human breast lesions ranging from normal to invasive carcinoma by using dual staining immunofluorescence. We found that in normal breast there was a strong positive association between ER and cyclin D 1 expression. In contrast there was a strong negative association between cyclin D 1 and Ki67 expression. Similar findings were seen for the other precancerous and cancerous breast lesions. Thus immunodetectable cyclin D 1 within individual cells does not appear to be associated with cell cycle progression in the benign or malignant breast but instead may have important interactions with ER. © 2001 Cancer Research Campaign http://www.bjcancer.com