G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus.
G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus.
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DOI：
10.1038/ncomms5366
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发表时间：
2014-07-09
影响因子：
16.6
通讯作者：
De Bolle, Xavier
中科院分区：
文献类型：
--
作者：
Deghelt, Michael;Mullier, Caroline;Sternon, Jean-Francois;Francis, Nayla;Laloux, Geraldine;Dotreppe, Delphine;Van der Henst, Charles;Jacobs-Wagner, Christine;Letesson, Jean-Jacques;De Bolle, Xavier
一些细胞内病原体，如流产布鲁氏菌，表现为双期感染过程，从性质不明的非增殖阶段开始。在此，我们从单细胞水平、HeLa细胞和巨噬细胞的培养和感染过程中研究了流产芽孢杆菌的细胞周期。两条细菌染色体的分离和复制位点的定位表明，大多数细菌细胞在被宿主细胞内吞液泡吞噬后立即新生。这些细菌细胞在接下来的4到6小时内不会启动DNA复制，这表明G1阻滞。此外，在此期间，生长完全停止，反映了全球细胞周期阻滞。生长和DNA复制稍后恢复，尽管细菌仍然居住在内核体样区室中。我们假设，在感染群体中，g1捕获的细菌占主导地位，以及细菌内化后的细胞周期捕获，可能构成细胞内病原体定殖新的增殖生态位的普遍策略。某些致病菌，如流产布鲁氏菌，在感染宿主细胞后会经历暂时的非增殖阶段。在这里，作者研究了B. abortus在感染期间的染色体复制和分离，表明非增殖阶段由g1捕获的新生细胞组成。
Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches. Certain pathogenic bacteria such as Brucella abortus undergo a temporary non-proliferative stage after infecting host cells. Here, the authors study chromosomal replication and segregation in B. abortus during infection, showing that the non-proliferative stage consists of G1-arrested newborn cells.
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