Grouper ATF1 plays an antiviral role in response to iridovirus and nodavirus infection.
Grouper ATF1 plays an antiviral role in response to iridovirus and nodavirus infection.
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DOI：
10.1016/j.fsi.2022.09.043
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发表时间：
2022-09
影响因子：
4.7
通讯作者：
Xinshuai Li;Jianling Huang;Cuiyu Liu;Jinpeng Chen;Shaowen Wang;Shina Wei;Min Yang;Q. Qin
中科院分区：
文献类型：
--
作者：
Xinshuai Li;Jianling Huang;Cuiyu Liu;Jinpeng Chen;Shaowen Wang;Shina Wei;Min Yang;Q. Qin
转录因子ATF1是CREB亚家族中ATF/CREB家族的成员，在哺乳动物中参与肿瘤发生、器官发育、生殖、细胞存活和凋亡等生理过程。然而，关于ATF1在鱼类中的研究报道相对较少，特别是关于其在鱼类抗病毒免疫中的作用。本研究克隆并鉴定了橙斑石斑鱼的ATF1（命名为EcATF1）。分子表征分析表明，EcATF1编码一个307个氨基酸的蛋白，包含PKID和bZIP_CREB1结构域。同源性分析表明，与a的同源性最高。飞刀(88.93%)。组织表达模式显示，EcATF1广泛分布于12个选定的组织中，在皮肤、鳃、肝脏和脾脏中表达量较高。亚细胞定位分析表明，EcATF1分布在GS细胞的细胞核中。EcATF1过表达抑制新加坡石斑鱼虹膜病毒（SGIV）和红斑石斑鱼神经坏死病毒（RGNNV）的复制，SGIV和RGNNV诱导的CPE程度降低，病毒基因转录水平和病毒衣壳蛋白表达水平降低。此外，EcATF1过表达上调干扰素通路相关基因和促炎因子，增加IFN、IFN刺激反应元件（ISRE）和核因子κB（NFκB）启动子活性。同时，EcATF1过表达正调控MHC-I信号通路，上调MHC-I启动子活性。综上所述，这些数据表明EcATF1在宿主抗病毒免疫反应中发挥重要作用。这项研究为ATF1在低等脊椎动物免疫系统中的功能提供了新的见解。
Transcription factor ATF1 is a member of the ATF/CREB family of the CREB subfamily and is involved in physiological processes such as tumorigenesis, organ development, reproduction, cell survival, and apoptosis in mammals. However, studies on ATF1 in fish have been relatively poorly reported, especially on its role in antiviral immunity in fish. In this study, ATF1 from orange-spotted grouper (named EcATF1) were cloned and characterized. Molecular characterization analysis showed that EcATF1 encodes a 307-amino-acid protein, containing PKID and bZIP_CREB1 domains. Homology analysis showed that had the highest homology withE. lanceolatus(88.93%). Tissue expression pattern showed that EcATF1 was extensively distributed in twelve selected tissues, with higher expression in the skin, gill, liver and spleen. Subcellular localization analysis showed that EcATF1 was distributed in the nucleus of GS cells. EcATF1 overexpression inhibits Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV) replication, as evidenced by a diminished degree of CPE induced by SGIV and RGNNV and a reduction in the level of viral gene transcription and viral capsid protein expression. Furthermore, EcATF1 overexpression upregulated interferon pathway-related genes and proinflammatory factors, and increased the promoter activities of IFN, IFN stimulated response element (ISRE), and nuclear factor κB(NFκB). Meanwhile, EcATF1 overexpression positive regulate the MHC-I signaling pathway, and upregulated the promoter activity of MHC-I. Collectively, these data demonstrate that EcATF1 plays an important role during the host antiviral immune response. This study provides insights into the function of ATF1 in the immune system of lower vertebrates.