Species-specific PCR primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans.
Species-specific PCR primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans.
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rDNA-ITS 区域中的物种特异性 PCR 引物作为 Serpula lacrymans 的诊断工具。
DOI：
10.1017/s0953756299001562
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发表时间：
2000
期刊：
影响因子：
2.5
通讯作者：
U. Moreth
中科院分区：
文献类型：
--
作者：
O. Schmidt;U. Moreth
本文对裂萼蛇螺（Serpula lacrymans）和裂萼蛇螺（S.通过PCR扩增himantioides以评估该DNA区域作为诊断工具。对扩增的ITS区进行测序，结果表明，S. lacrymans和S. himantioides。利用两种真菌的碱基序列差异设计了两种真菌的种特异性寡核苷酸PCR引物。对于实验室和现场样品，每种引物都选择性地检测其靶生物。与引起室内木材腐烂的其它真菌无交叉反应。
The ITS of the nuclear ribosomal DNA of Serpula lacrymans and S. himantioides was amplified by PCR to evaluate this DNA region as a diagnostic tool. Sequencing of the amplified ITS region revealed a size of the complete ITS of 654 bp for S. lacrymans and 650 bp for S. himantioides. The base sequence difference between the two fungi was used to design species-specific oligonucleotide PCR primers for both species. With laboratory and field samples, each primer selectively detected its target organism. No cross reaction occurred with other fungi causing rot of indoor wood.