Characterization of pancreatic endocrine cells of the European common frog Rana temporaria.
Characterization of pancreatic endocrine cells of the European common frog Rana temporaria.
复制标题
欧洲青蛙胰腺内分泌细胞的表征。
DOI：
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发表时间：
2000
影响因子：
2.7
通讯作者：
A. Villaro
中科院分区：
文献类型：
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作者：
J. Etayo;L. Montuenga;P. Sesma;O. Díaz de Rada;J. Rovira;A. Villaro
为了表征蛙胰腺的内分泌细胞类型，常规染色，银浸渍和免疫细胞化学方法的光镜和电镜已被应用于石蜡，薄切片和半薄切片，其中许多是连续对。还报告了每种内分泌细胞类型的频率和分布（岛状、岛外分泌细胞或胰管内）的定量数据。已鉴定出四种不同的内分泌细胞类型：胰岛素（B）细胞，其也对[Met]脑啡肽具有免疫反应性;胰高血糖素/PP（A/PP）细胞，其也对GLP 1具有免疫反应性;生长抑素（D）细胞;以及第四种内容和功能未知的内分泌样细胞类型（X细胞）。X细胞显示特征性的超微结构和着色特征，但对所有37种抗血清均呈非免疫反应性。[Met]脑啡肽在两栖动物胰腺内分泌细胞中的存在是首次报道。几乎一半（44.9 +/- 7.9）的总内分泌细胞群位于胰岛外，主要分布在外分泌细胞中。约37.2 +/- 4.6%的总内分泌细胞群对胰岛素呈免疫反应性，48.8 +/- 6.9%对胰高血糖素/PP呈免疫反应性，14.0 +/- 4.9%对生长抑素呈免疫反应性;在外分泌实质内发现79.2 +/- 6.4%的胰高血糖素/PP细胞，代表大部分（86.4 +/- 4.3%）岛外内分泌组分。相反，大多数B细胞（94.2 +/- 2.1%）位于胰岛内; 30.8 +/- 12.9%的D细胞位于胰岛外。
To characterize the endocrine cell types of the pancreas of Rana temporaria, conventional staining, silver impregnation, and immunocytochemical methods for light and electron microscopy have been applied to paraffin, thin and semithin sections, many of them serial pairs. Quantitative data on the frequency and distribution (insular, extrainsular among the exocrine cells, or within the pancreatic ducts) of each endocrine cell type are also reported. Four distinct endocrine cell types have been identified: insulin (B) cells, which are also immunoreactive for [Met]enkephalin; glucagon/PP (A/PP) cells, also immunoreactive for GLP1; somatostatin (D) cells; and a fourth endocrine-like cell type (X cells) of unknown content and function. X cells display characteristic ultrastructure and tinctorial traits but are nonimmunoreactive for all of the 37 antisera tested. The presence of [Met]enkephalin in amphibian pancreatic endocrine cells is now reported for the first time. Almost half (44.9 +/- 7.9) of the total endocrine cell population lies outside the islets, mainly spread among the exocrine cells. Approximately 37.2 +/- 4.6% of the total endocrine cell population was immunoreactive for insulin, 48.8 +/- 6.9% was immunoreactive for glucagon/PP, and 14.0 +/- 4.9% was immunoreactive for somatostatin; 79.2 +/- 6.4% of glucagon/PP cells are found within the exocrine parenchyma, representing the majority (86.4 +/- 4.3%) of extrainsular endocrine component. On the contrary, most B cells (94.2 +/- 2.1%) are located within the islets; 30.8 +/- 12.9% of D cells are found outside the islets.
负鼠胰岛素、胰高血糖素和胰多肽：氨基酸序列。
影响因子：
3
作者：
Yu,JH;Eng,J;Rattan,S;Yalow,RS
通讯作者：
Yalow,RS