Isolation and characterization of autotrophic, hydrogen-utilizing, perchlorate-reducing bacteria
Isolation and characterization of autotrophic, hydrogen-utilizing, perchlorate-reducing bacteria
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DOI：
10.1007/s00253-004-1725-0
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发表时间：
2005-04-01
影响因子：
5
通讯作者：
Parkin, GF
中科院分区：
文献类型：
--
作者：
Shrout, JD;Scheetz, TE;Parkin, GF
近年来的研究表明，在氢气的作用下，一些纯培养和混合培养的细菌可以降解高氯酸盐（ClO 4-）。本文报道了两株能利用无机碳生长的高氯酸盐降解菌的分离。这些自养细菌属于脱氯单胞菌属，是第一个微需氧的脱氯单胞菌属物种，在大气氧浓度下不能生长。Dechloromonasp.JDS5和Dechloromonasp.JDS6是第一个从高氯酸盐污染场地中分离到的降解高氯酸盐的自养菌。测得的氢阈值高于其他环境显著的、利用氢的厌氧细菌（例如，halorespirers）。这些细菌的β-歧化酶的活性是更大的自养生长的细胞比异养生长的细胞上的乳酸。这些细菌使用富马酸盐作为替代电子受体，这是高氯酸盐还原菌在有机电子受体上生长的第一个报告。
Recent studies have shown that perchlorate (ClO4-) can be degraded by some pure-culture and mixed-culture bacteria with the addition of hydrogen. This paper describes the isolation of two hydrogen-utilizing perchlorate-degrading bacteria capable of using inorganic carbon for growth. These autotrophic bacteria are within the genus Dechloromonas and are the first Dechloromonas species that are microaerophilic and incapable of growth at atmospheric oxygen concentrations. Dechloromonas sp. JDS5 and Dechloromonas sp. JDS6 are the first perchlorate-degrading autotrophs isolated from a perchlorate-contaminated site. Measured hydrogen thresholds were higher than for other environmentally significant, hydrogen-utilizing, anaerobic bacteria (e.g., halorespirers). The chlorite dismutase activity of these bacteria was greater for autotrophically grown cells than for cells grown heterotrophically on lactate. These bacteria used fumarate as an alternate electron acceptor, which is the first report of growth on an organic electron acceptor by perchlorate-reducing bacteria.