DNA topology of the ordered chromatin domain 5' to the human c-myc gene.
DNA topology of the ordered chromatin domain 5' to the human c-myc gene.
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人类 c-myc 基因 5 端有序染色质结构域的 DNA 拓扑。
DOI：
10.1093/nar/17.7.2819
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发表时间：
1989
影响因子：
14.9
通讯作者：
Leffak,M
中科院分区：
文献类型：
--
作者：
Kumar,S;Leffak,M
位于人霉素基因5′端的DNA限制性片段在聚丙烯酰胺凝胶上显示异常的电泳迁移率。对myc侧翼DNA的计算机建模表明，跨越核苷酸-1690到-1054（相对于c-mycpromoter P1）和-718到-452的缓慢移动的DNA片段形成了大型的左手超螺旋或弯曲结构，而跨越核苷酸-407到+78的快速移动的DNA片段具有异常的直结构。这些分析还预测通过超螺旋域弯曲的局部区域的周期性阵列。分离的细胞核的微球菌核酸酶消化揭示，缓慢移动的DNA片段存在于一个有序的染色质结构稳定的核酸酶，而快速移动的DNA片段的消化模式表明一个不太有序的阵列的核小体或非核小体染色质结构。
DNA restriction fragments located 5′ to the humanc-mycgene display anomalous electrophoretic mobility on polyacrylamide gels. Computer modeling of thec-mycflanking DNA suggests that the slow-moving DNA fragments spanning nucleotides −1690 to −1054 (relative toc-mycpromoter P1) and −718 to −452 form large left handed superhelices or curved structures while the fast-moving DNA fragment spanning nucleotides −407 to + 78 has an unusually straight structure. These analyses also predict a periodic array of localized regions of bending through the superhelical domains. Microccocal nuclease digestion of isolated nuclei reveals that the slow-moving DNA fragments exist in an ordered chromatin structure stable to nuclease, whereas the digestion pattern of the fast-moving DNA fragment suggests a less ordered array of nucleosomes or a non-nucleosomal chromatin structure.