Thyroid hormone-induced chondrocyte terminal differentiation in rat femur organ culture
Thyroid hormone-induced chondrocyte terminal differentiation in rat femur organ culture
复制标题
甲状腺激素诱导大鼠股骨器官培养中软骨细胞终末分化
DOI：
10.1007/s004410051127
复制
发表时间：
1998
影响因子：
3.6
通讯作者：
M. Itoman
中科院分区：
文献类型：
--
作者：
R. Wakita;T. Izumi;M. Itoman
甲状腺激素在骨骼成熟中起作用。本研究旨在探讨甲状腺激素对大鼠股骨器官培养系统骨骺软骨生长成熟的影响。在没有血清的情况下，随着时间的推移，股骨的重量和大小都增加了。甲状腺素治疗抑制了生长速度。甲状腺素诱导股骨髁关节面附近区域软骨细胞肥大。在培养21天后，通过免疫组织化学和逆转录/聚合酶链反应检测X型胶原的表达，X型胶原是软骨细胞终末分化的标志。这些数据表明甲状腺素通过刺激软骨细胞终末分化来抑制软骨生长。
Thyroid hormone plays a role in skeletal maturation. The present study was undertaken to investigate the effect of thyroid hormone on growth and maturation of the epiphyseal cartilage in the rat femur organ culture system. The femora increased in both weight and size over time in culture in the absence of serum. The growth rate was suppressed by thyroxine treatment. Thyroxine induced chondrocyte hypertrophy in the area adjacent to the articular surface in the femoral condyle. In this area, the expression of type X collagen, a marker of chondrocyte terminal differentiation, was detected after 21 days in culture by immunohistochemistry and reverse transcription/polymerase chain reaction. These data suggest that thyroxine suppresses cartilage growth by stimulating chondrocyte terminal differentiation.