MOLECULAR-CLONING AND ANALYSIS OF A NEW VARIANT OF HUMAN T-CELL LEUKEMIA-VIRUS (HTLV-IB) FROM AN AFRICAN PATIENT WITH ADULT T-CELL LEUKEMIA-LYMPHOMA
MOLECULAR-CLONING AND ANALYSIS OF A NEW VARIANT OF HUMAN T-CELL LEUKEMIA-VIRUS (HTLV-IB) FROM AN AFRICAN PATIENT WITH ADULT T-CELL LEUKEMIA-LYMPHOMA
复制标题
DOI：
10.1002/ijc.2910340505
复制
发表时间：
1984-01-01
影响因子：
6.4
通讯作者：
WONGSTAAL, F
中科院分区：
文献类型：
--
作者：
HAHN, BH;SHAW, GM;WONGSTAAL, F
我们报告了一名非洲成人 T 细胞白血病/淋巴瘤 (ATL) 患者中 HTLV-I 新变种的鉴定和特征分析。通过 Southern 印迹分析，在从该患者的外周血淋巴细胞 (PBL) 和淋巴结细胞建立的三个 T 细胞系中检测到前病毒序列。我们对其中两种细胞系的原病毒进行了分子克隆和分析，一种是通过直接培养 PBL 建立的，另一种是通过 PBL 与脐带血 T 细胞共培养建立的。这两个 HTLV 克隆包含全长原病毒，其 44 个限制性酶切位点中有 44 个彼此相同。它们与原型 HTLV-I 密切相关，但又不同，其包膜和 5' pX 区域存在分歧，因此代表了 HTLV-I 的新变体。我们将其命名为 HTLV-Ib。然而，尽管存在基因组差异，HTLV-Ib 保留了其对 OKT4+ 淋巴细胞的趋向性以及启动和维持这些细胞转化的能力。在该非洲 ATL 患者中发现 HTLV-I 变异，以及最近的血清流行病学研究结果，将先前在加勒比海和日本发现的 HTLV-I 相关恶性肿瘤的患病率扩展到非洲大陆。
We report the identification and characterization of a new variant of HTLV‐I in an African patient with adult T‐cell leukemia/lymphoma (ATL). Proviral sequences were detected by Southern blot analysis in three T‐cell lines established from this patient's peripheral blood lymphocytes (PBL) and lymph‐node cells. We molecularly cloned and analyzed proviruses from two of these cell lines, one established by direct culture of PBL and one established by co‐cultivation of PBL with cord‐blood T cells. These two HTLV clones contained full‐length proviruses which were identical to each other in 44 out of 44 restriction enzyme sites. They were closely related to, but distinct from, the prototype HTLV‐I, having divergence in their envelope and 5′ pX regions and therefore represented a new variant of HTLV‐I. We designated it as HTLV‐Ib. Despite the genomic differences, however, HTLV‐Ib retained its tropism for OKT4+ lymphocytes as well as its ability to initiate and maintain transformation of these cells. The finding of a variant of HTLV‐I in this African ATL patient, along with the results of recent seroepidemiological studies, extends to the African continent the prevalence of HTLV‐I associated malignancy previously identified in the Caribbean and Japan.