The distribution of spindle microtubules during mitosis in cultured human cells.
The distribution of spindle microtubules during mitosis in cultured human cells.
复制标题
培养的人类细胞有丝分裂过程中纺锤微管的分布。
DOI：
10.1083/jcb.49.2.468
复制
发表时间：
1971-05-01
影响因子：
7.8
通讯作者：
Landis, S C
中科院分区：
文献类型：
--
作者：
McIntosh, J R;Landis, S C
有丝分裂中的 WI-38 和 HeLa 细胞是从固定的单层培养物中选出的，并连续切片用于电子显微镜检查。垂直于主轴轴线的截面允许对主轴轴线上每个位置处的微管数量进行计数，从而制备微管分布轮廓。讨论了该方法固有的错误。从一个有丝分裂阶段到另一个有丝分裂阶段的小管分布的变化提供了有关有丝分裂期间纺锤体小管行为的证据。中期板上通过染色体的小管数量与每半纺锤体最大数量之比约为1/2。该比率在后期早期变化不大，然后在后期后期降低，大约在同一时间，在区间中部出现小管数量增加的区域。茎体形成的区域包含的小管数量约为层间其他地方可见的小管数量的 3/2。当中体在末期形成时，该比率几乎是恒定的，然后在胞质分裂的最后阶段发生之前在早期间期增加到 2/1。
WI-38 and HeLa cells in mitosis have been selected from fixed monolayer cultures and serially sectioned for electron microscopy. Sections perpendicular to the spindle axis permit counting of the number of microtubules at each position on the spindle axis and hence the preparation of tubule distribution profiles. Errors intrinsic to this method are discussed. The changes in the tubule distributions from one mitotic stage to another provide evidence concerning the behavior of the spindle tubules during mitosis. The ratio of the number of tubules passing the chromosomes on the metaphase plate to the maximum number in each half spindle is about 1/2. This ratio changes little in early anaphase, and then decreases in late anaphase at about the same time that a zone of increased tubule number develops at the middle of the interzone. The region where the stem bodies form contains about 3/2 the number of tubules seen elsewhere in the interzone. This ratio is almost constant as the mid-body forms in telophase and then increases to 2/1 in early interphase before the final stages of cytokinesis occur.