Larval sensilla of the moth Heliothis virescens respond to sex pheromone components
Larval sensilla of the moth Heliothis virescens respond to sex pheromone components
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DOI：
10.1111/imb.12253
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发表时间：
2016-10
影响因子：
2.6
通讯作者：
M. Zielonka;P. Gehrke;E. Badeke;S. Sachse;H. Breer;J. Krieger
中科院分区：
文献类型：
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作者：
M. Zielonka;P. Gehrke;E. Badeke;S. Sachse;H. Breer;J. Krieger
雌性释放的性信息素控制着飞蛾的交配行为。最近的研究表明，性信息素不仅能吸引成年雄虫，还能吸引毛毛虫。单感器记录显示，Heliothis virescens幼虫触角感器对特定的性信息素成分有反应。为了寻找幼虫信息素检测的分子基础，我们发现幼虫触角上的嗅觉感受器具有与成年雄蛾相同的介导性信息素检测的分子元件，包括Heliothis virescens受体6 （HR6）和HR13，以及感觉神经元膜蛋白1 （SNMP1）。在三个大的基本感受器中鉴定出38个嗅感觉神经元；其中6个基于SNMP1的表达被认为是候选信息素应答细胞。信息素受体HR6在2个细胞中表达，受体HR13在3个细胞中表达。这些假设的信息素反应神经元伴随着表达信息素结合蛋白1 （PBP1）和PBP2的细胞。结果表明，幼虫感受器对雌性发出的性信息素的反应与成年雄性的触角是基于相同的分子机制。
Female‐released sex pheromones orchestrate the mating behaviour of moths. Recent studies have shown that sex pheromones not only attract adult males but also caterpillars. Single sensillum recordings revealed that larval antennal sensilla of the moth Heliothis virescens respond to specific sex pheromone components. In search for the molecular basis of pheromone detection in larvae, we found that olfactory sensilla on the larval antennae are equipped with the same molecular elements that mediate sex pheromone detection in adult male moths, including the Heliothis virescens receptors 6 (HR6) and HR13, as well as sensory neurone membrane protein 1 (SNMP1). Thirty‐eight olfactory sensory neurones were identified in three large sensilla basiconica; six of these are considered as candidate pheromone responsive cells based on the expression of SNMP1. The pheromone receptor HR6 was found to be expressed in two cells and the receptor HR13 in three cells. These putative pheromone responsive neurones were accompanied by cells expressing pheromone‐binding protein 1 (PBP1) and PBP2. The results indicate that the responsiveness of larval sensilla to female‐emitted sex pheromones is based on the same molecular machinery as in the antennae of adult males.