Identification and characterization of proximal 6q deletions in prostate cancer.
Identification and characterization of proximal 6q deletions in prostate cancer.
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发表时间：
1996-09
期刊：
影响因子：
11.2
通讯作者：
K. Cooney;J. Wetzel;Christina M. Consolino;K. Wojno
中科院分区：
文献类型：
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作者：
K. Cooney;J. Wetzel;Christina M. Consolino;K. Wojno
8p、10q、13q、16q和18q的等位基因缺失在前列腺癌中经常被证实，这意味着在这些区域可能存在肿瘤抑制基因。然而，可能有一些额外的遗传事件定义从正常前列腺上皮到前列腺癌的进展尚未确定。为了描述散发性前列腺癌中一个新的缺失区域，我们使用6号染色体上的10个多态性标记分析了52例根治性前列腺切除术患者的肿瘤的杂合性缺失（LOH），其中6号染色体上有1个标记，6号染色体上有9个标记。从现有数据库中选择标记，构建综合的连锁图谱。通过本分析，52例散发性前列腺癌中有17例（33%）检测到一个或多个多态性标记的LOH。17个肿瘤中有13个具有从D6S286到D6S283或6q14-21的共同等位基因缺失区域，其中包含标记物D6S1082和D6S501的最小缺失区域。第二个单独的缺失区域位于标记D6S404周围。一个或多个6q标记物的LOH与Gleason分级或癌症的病理分期无关。总之，这是第一次对前列腺癌中6q缺失的综合分析，我们得出结论，6q14-21可能含有一个在前列腺癌发生中重要的肿瘤抑制基因。
Allelic loss of 8p, 10q, 13q, 16q, and 18q has been frequently demonstrated in prostate cancer, implying the existence of putative tumor suppressor genes in these regions. However, there are likely a number of additional genetic events that define the progression from normal prostatic epithelium to prostate cancer that have yet to be identified. To characterize a novel region of deletion in sporadic prostate cancers, 52 tumors obtained from radical prostatectomy cases were analyzed for loss of heterozygosity (LOH) using 10 polymorphic markers spanning chromosome 6 including one marker on 6p and nine markers on 6q. Markers were selected from available databases, and a comprehensive linkage map was constructed. By this analysis, LOH for one or more polymorphic markers was detected in 17 of 52 sporadic prostate cancer cases (33%). Thirteen of 17 tumors were shown to have a common region of allelic loss extending from D6S286 to D6S283 or 6q14-21, with a minimum region of loss containing markers D6S1082 and D6S501. A second separate region of deletion centered around marker D6S404. LOH of one or more 6q markers did not correlate with Gleason grade or pathological stage of the cancer. In summary, this is the first comprehensive analysis of 6q deletions in prostate cancer, and we conclude that 6q14-21 may harbor a tumor suppressor gene important in prostate carcinogenesis.