EXPRESSION OF THE RAT ALDOLASE-B GENE - A LIVER-SPECIFIC PROXIMAL PROMOTER AND AN INTRONIC ACTIVATOR
EXPRESSION OF THE RAT ALDOLASE-B GENE - A LIVER-SPECIFIC PROXIMAL PROMOTER AND AN INTRONIC ACTIVATOR
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DOI：
10.1016/s0006-291x(05)80244-x
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发表时间：
1991-04-30
影响因子：
3.1
通讯作者：
PICHARD, AL
中科院分区：
文献类型：
--
作者：
GREGORI, C;GINOT, F;PICHARD, AL
研究了调控大鼠醛缩酶B基因表达的顺式作用DNA序列的性质和位置。检测到两个肝脏特异的DNase I超敏感位点，一个位于帽状位点的上游，第二个位于第一个4.8kbp长的内含子的中间。帽基因5‘端190个碱基的片段是组织特异性的弱核心启动子：它在Hep G2细胞和肝细胞中引导了可检测到的报告基因的表达，但在成纤维细胞中不表达。当构建的载体包含完整的第一内含子时，组织特异性表达至少被刺激16倍。内含子激活序列可归因于位于下游肝脏特异的DNase I超敏部位的内侧2kbp片段。
The nature and location of the cis-acting DNA sequences regulating expression of the rat aldolase B gene has been investigated. Two liver-specific DNAse I hypersensitive sites were detected, one located just upstream from the cap site, the second in the middle of the first, 4.8-kbp-long, intron. A fragment of 190 bp 5′ to the cap site behaved as a tissue-specific but weak core promoter: it directed a detectable reporter gene expression in the Hep G2cells and hepatocytes, but not in fibroblasts. The tissue-specific expression was stimulated at least 16 fold when constructs contained the entire first intron. The intronic activating sequences could be ascribed to an inner 2 kbp fragment in which the downstream liver-specific DNAse I hypersensitive site was located.