FIBROBLAST LINES EXPRESSING ACTIVATED C-MYC ONCOGENES ARE TUMORIGENIC IN NUDE-MICE AND SYNGENEIC ANIMALS
FIBROBLAST LINES EXPRESSING ACTIVATED C-MYC ONCOGENES ARE TUMORIGENIC IN NUDE-MICE AND SYNGENEIC ANIMALS
复制标题
DOI：
10.1016/0092-8674(84)90012-6
复制
发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
COLE, MD
中科院分区：
文献类型：
--
作者：
KEATH, EJ;CAIMI, PG;COLE, MD
通过转染与病毒启动子连接的c-myc基因，研究c-myc表达对成纤维细胞生长和形态的影响。转染NIH/3 T3或Rat 2细胞后未观察到病灶。使用SV 2-neo共选择建立了含有激活的c-myc基因的细胞系，并研究了细胞的几个生长参数。在软琼脂中，细胞的增殖能力略有增加，形成集落的效率仅为1- 2%。转染c-myc的细胞在0.5%血清中生长良好，而对照组则不能。观察到的细胞生长的主要差异是，c-myc转染的细胞接种到裸鼠或同系大鼠中时具有致瘤性。对来自致瘤细胞的RNA的分析显示，来自转染基因的c-myc表达水平比来自内源基因的c-myc表达水平高2-6倍。成纤维细胞肿瘤中c-mys RNA的水平与小鼠浆细胞瘤中发现的水平相似。内源性c-myc基因的表达受转染基因的影响，在培养中的亚融合细胞，但该基因被关闭在裸鼠肿瘤。组成型c-myc表达导致永生化细胞系的致瘤性。
The influence of c-myc expression on fibroblast growth and morphology was investigated by transfection of c-myc genes linked to viral promoters. No foci were observed after transfection of NIH/3T3 or Rat 2 cells. Cell lines containing activated c-myc genes were established using SV2-neo coselection and several growth parameters of the cells were studied. The cells showed a slight increase in refractility and fomred colonies in soft agar with an efficiency of only 1-2%. The c-myc-transfected cells grew well in 0.5% serum, while the controls did not. The major difference in cell growth noted was that c-myc-transfected cells were tumorigenic when inoculated into nude mice or syngeneic rats. Analysis of RNA from the tumorigenic cells showed a level of c-myc expression from the transfected genes that was 2-6 fold higher than that from the endogenous gene. The level of c-mys RNA in the fibroblast tumors was similar to that found in mouse plasmacytomas. Expression of the endogenous c-myc gene was affected by the transfected genes for subconfluent cells in culture, but the gene was shut off in the nude mouse tumors. Constitutive c-myc expression leads to tumorigenicity in immortalized cell lines.