FOLLICULAR FLUID FROM HUMAN OVARIES CONTAINS PLASMA KALLIKREIN IN FREE AND IN COMPLEX WITH α 2 -MACROGLOBULIN
FOLLICULAR FLUID FROM HUMAN OVARIES CONTAINS PLASMA KALLIKREIN IN FREE AND IN COMPLEX WITH α 2 -MACROGLOBULIN
复制标题
来自人类卵巢的卵泡液含有游离血浆激肽释放酶和与 α 2 -巨球蛋白复合的血浆激肽释放酶
DOI：
10.2220/biomedres.18.161
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发表时间：
1997
影响因子：
1.2
通讯作者：
Takayuki Takahashi
中科院分区：
文献类型：
--
作者：
J. Ohnishi;M. Murata;K. Kohyama;H. Yoshida;S. Wada;S. Makinoda;S. Fujimoto;Takayuki Takahashi
Follicular fluid from human ovaries obtained in in vitro fertilization procedures contained substantial proteinase activities toward synthetic, arginine-containing endopeptidase substrates. Using Pro-Phe-Arg-4-methylcoumary1-7-amide as a marker for activity, two enzymes, designated human fluid serine proteinase-1 (hFSP-1) and -2 (hFSP-2), were isolated. The molecular weights of intact hFSP-1 and hFSP-2 were estimated to be approximately 90,000 and 730,000, respectively. They exhibited virtually identical substrate specificities and inhibition profiles when tested for substrates and inhibitors with low molecular weight. Polypeptide proteinase inhibitors, such as soybean trypsin inhibitor and aprotinin, strongly inhibited hFSP-1, but only inhibited hFSP-2 to a limited extent. These results, together with those of electrophoretic and immunological characteri-zation, revealed that hFSP-1 and hFSP-2 are free plasma kallikrein and the enzyme in complex with the plasma inhibitor 0:2-macroglobulin, respectively. hFSP-1 activated human sin-gle-chain precursor tissue-type plasminogen activator. This study also indicates that the plasma kallikrein present in follicular fluid probably comes from the liver.
