Inhibition of iron uptake by phytic acid, tannic acid, and ZnCl2:: Studies using an in vitro digestion/Caco-2 cell model
Inhibition of iron uptake by phytic acid, tannic acid, and ZnCl2:: Studies using an in vitro digestion/Caco-2 cell model
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DOI：
10.1021/jf011046u
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发表时间：
2002-01-16
影响因子：
6.1
通讯作者：
Miller, DD
中科院分区：
文献类型：
--
作者：
Glahn, RP;Wortley, GM;Miller, DD
本研究的目的是在体外消化/Caco-2细胞培养模型中记录植酸、单宁酸和锌对铁吸收的影响。植酸和单宁酸对铁吸收的影响进行了测量，在增加的摩尔比的氯化铁植酸或单宁酸。植酸对铁吸收的最大抑制发生在铁与植酸的比例为1：10时。可透析铁降低与低铁植酸比，但增加与铁：植酸比大于1：3，表明更多的铁是可溶性的，在较高的植酸水平，但可用性较低。在人体研究中，血红素铁被植酸抑制的程度低于非血红素铁。单宁酸是一个更有效的抑制剂的非血红素铁的吸收，最大的抑制（97.5%）的铁的吸收发生在1：1或更少的比例。添加ZnCl 2的比例为1：0.5和1：1的消化液中的铁的吸收分别减少了57%和80%。总体而言，结果与人体研究定性一致，并证明了这些化合物对该模型系统中铁吸收的相对影响。这项研究为确定更复杂膳食条件下的铁可用性提供了关键信息。
The objective of this study was to document the effects of phytic acid, tannic acid, and zinc on iron uptake in an in vitro digestion/Caco-2 cell culture model. The effects of phytic acid and tannic acid on iron uptake were measured at increasing molar ratios of FeCl3 to phytic acid or tannic acid. Maximal inhibition of iron uptake by phytic acid occurred at a 1:10 ratio of Fe to phytic acid. Dialyzable Fe decreased with a low Fe to phytic acid ratio but increased with Fe:phytic acid ratios greater than 1:3 indicating that more iron was soluble at higher phytic acid levels but less available. As in human studies, heme iron was less inhibited by phytic acid than nonheme iron. Tannic acid was a more potent inhibitor of nonheme iron uptake, as maximal inhibition (97.5%) of iron uptake occurred at a ratio of 1:1 or less. The addition of ZnCl2 to the digest at ratios of 1:0.5 and 1:1 decreased iron uptake by 57 and 80%, respectively. Overall, the results agree qualitatively with studies in humans and demonstrate the relative effects of these compounds on iron uptake in this model system. This study provides key information for determining iron availability under more complex meal conditions.