Influence of cell adhesive molecules attached onto PEG-lipid-modified fluid surfaces on cell adhesion
Influence of cell adhesive molecules attached onto PEG-lipid-modified fluid surfaces on cell adhesion
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DOI：
10.1016/j.colsurfb.2018.12.015
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发表时间：
2019-03-01
影响因子：
5.8
通讯作者：
Teramura, Yuji
中科院分区：
文献类型：
--
作者：
Noiri, Makoto;Kushiro, Keiichiro;Teramura, Yuji
细胞间相互作用在各种生物事件中的参与表明了使用流体模型表面研究细胞-细胞相互作用的重要性。在这里，我们提出了一种由自组装单分子膜(SAM)和聚乙二醇偶联磷脂(PEGLID)衍生物组成的流体表面，它可以作为支撑类脂膜的替代品。通过石英晶体消散微天平(QCM-D)和光漂白后荧光恢复(FRAP)定量测定了不同迁移率的流体表面。制备了聚乙二醇脂类化合物的扩散系数在0.9+/-0.25~0.16+/-0.03µm(2)/s之间的不同类型的流体表面，并将精氨酸甘氨酰天冬氨酸(RGD)多肽固定在其上进行细胞黏附，并与相同表面密度的RGD多肽固体表面进行了比较。液体表面显示，在流动性较高的表面不能形成上皮细胞(MCF-10A)和人脐静脉内皮细胞(HUVEC)的细胞黏附，而细胞可以黏附在流动性较低的表面(聚乙二醇脂的侧向扩散约为其20倍)和固体表面。有趣的是，附着在流动性较低的表面的细胞以正常的速度增殖，同时保持其圆形形态，这与在固体表面观察到的形状不同。因此，支架的流动性对细胞的黏附行为有很大的影响，是设计新型仿生生物医学支架的重要参数。
The involvement of intercellular interactions in various biological events indicates the importance of studying cell-cell interactions using fluid model surfaces. Here, we propose a fluid surface composed of a self-assembled monolayer (SAM) and poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) derivatives, which can be an alternative to supported lipid membranes. The modification of SAM surfaces with PEG-lipids carrying functional peptides resulted in the formation of the fluid surfaces with different mobility, which was quantitatively determined by quartz crystal microbalance with dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP). Different types of fluid surfaces with calculated diffusion coefficients between 0.9 +/- 0.25 and 0.16 +/- 0.03 mu m(2)/sec for PEG-lipids derivatives were fabricated, onto which arginylglycylaspartate (RGD) peptides were immobilized for cell adhesion, and compared to solid surfaces with the same surface density of RGD peptides. The fluid surfaces revealed that cell adhesions of epithelial cells (MCF-10 A) and human umbilical vein endothelial cells (HUVEC) could not be established on the surfaces with higher fluidity, while cells could adhere onto surfaces with lower fluidity, where the lateral diffusion of PEG-lipids was approximately 20 times lower, and solid surfaces. Interestingly, cells that adhered onto the surface with lower fluidity proliferated at a normal rate while maintaining their round morphology, which was a different shape from that observed on solid surfaces. Thus, the scaffold fluidity greatly influenced cell adhesion behaviors, demonstrating that it is an important parameter for designing novel biomimetic scaffolds for biomedical applications.