Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide.
Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide.
复制标题
用放线菌酮处理 G1 期同步中国仓鼠细胞中 DNA 合成的抑制。
DOI：
10.1016/0014-4827(71)90630-6
复制
发表时间：
1971
影响因子：
3.7
通讯作者：
D. P. Highfield
中科院分区：
文献类型：
--
作者：
M. Schneiderman;W. Dewey;D. P. Highfield
在同步化中国仓鼠卵巢细胞中，研究了蛋白质合成抑制剂放线菌酮（CHM）在G1期处理后DNA合成起始的延迟。在有丝分裂中处理1-5小时的细胞在DNA合成的起始中被CHM处理的长度延迟。然而，有丝分裂后处理的细胞延迟启动DNA合成的时间长度大于CHM处理的持续时间。延迟的长度和CHM处理的持续时间之间的差异达到最大值约4小时，并且大致与CHM处理的持续时间和添加CHM时G1中的时间成比例。当细胞进入S期时，处理使DNA合成停止;然而，去除CHM后，DNA合成立即恢复。这些结果表明，需要RNA和/或蛋白质合成的G1期事件参与DNA合成的起始和继续。在G1期蛋白质合成的抑制导致这些事件的衰减（平均寿命为2.9小时），并且已经衰减的事件必须在DNA合成开始之前重复。在前一个周期中发生的事件，或在加入CHM前3.5小时以上的G1中发生的事件，不必重复。在非常晚的G1期或早期S期的细胞中，导致DNA合成起始的所有事件已经完成，并且在CHM处理长达5小时的持续时间后不必重复。
The delay in the initiation of DNA synthesis after treatments during G1 with cycloheximide (CHM), an inhibitor of protein synthesis, has been studied in synchronous Chinese hamster ovary cells. Cells treated in mitosis for a duration of 1–5 h were delayed in the initiation of DNA synthesis by the length of the CHM treatment. However, cells treated after mitosis were delayed in the initiation of DNA synthesis by a length of time greater than the duration of the CHM treatment. The difference between the length of the delay and the duration of CHM treatment reached a maximum of about 4 h, and was roughly proportional to both the duration of the CHM treatment and the time in G1 when CHM was added. Treatment of cells as they entered S phase stopped DNA synthesis; however, after removal of CHM, DNA synthesis immediately resumed.These results suggest the following, G1 events requiring RNA and/or protein synthesis are involved in the initiation and continuation of DNA synthesis. Inhibition of protein synthesis during G1 results in the decay of these events (mean lifetime of 2.9 h), and the events which have decayed away must be repeated before DNA synthesis can be initiated. Events which occurred in the previous cycle, or in G1 more than 3.5 h prior to the addition of CHM, do not have to be repeated. In cells in very late G1 or early S phase, all the events leading to the initiation of DNA synthesis have been completed and do not have to be repeated following CHM treatments up to 5 h in duration.