DYNAMIC BEHAVIOR OF ENDOPLASMIC-RETICULUM IN LIVING CELLS
DYNAMIC BEHAVIOR OF ENDOPLASMIC-RETICULUM IN LIVING CELLS
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DOI：
10.1016/0092-8674(88)90177-8
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发表时间：
1988-07-01
期刊：
影响因子：
64.5
通讯作者：
CHEN, LB
中科院分区：
文献类型：
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作者：
LEE, C;CHEN, LB
用荧光碳菁染料DiOC 6（3）处理的活CV-1细胞，通过荧光显微镜研究内质网（ER）。使用视频记录和图像处理技术，几种不同形式的高度本地化的运动的ER记录，分类，并分析的机制和结构的影响。这些包括小管分支、环闭合和滑动。这些局部的运动已经被观察到产生ER的基本元素：线性小管，多边形网状结构，和三重连接。我们建议，因此，他们作为机制，构建多边形网格的相互连接的膜小管，构成ER。这些运动的性质表明可能涉及细胞骨架，并且，鉴于ER和微管分布的密切相关性，以及随附的论文（Dabora和Sheetz），微管可能在产生ER运动性以及构建和维持活细胞中的ER网络中发挥作用。
Endoplasmic reticulum (ER) was studied by fluorescence microscopy of living CV-1 cells treated with the fluorescent carbocyanine dye DiOC6(3). Using video recording and image processing techniques, several distinct forms of highly localized movements of ER were documented, categorized, and analyzed in terms of mechanism and structural implications. These include tubule branching, ring closure, and sliding. These localized movements have been obseved to generate the basic elements of ER: linear tubules, polygonal reticulum, and triple junctions. We propose that as such they act as the mechanism for constructing the polygonal lattice of interconnected membrane tubules that constitutes ER. The nature of these movements suggests possible involvement of the cytoskeleton, and, in view of the close correlations in the distributions of ER and microtubules, and the accompanying paper (Dabora and Sheetz), it is possible that microtubules may play a role in generating ER motility and in constructing and maintaining the ER network in living cells.