Composition of Acridid gut bacterial communities as revealed by 16S rRNA gene analysis
Composition of Acridid gut bacterial communities as revealed by 16S rRNA gene analysis
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DOI：
10.1016/j.jip.2007.09.010
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发表时间：
2008-03-01
影响因子：
3.4
通讯作者：
Charnley, A. K.
中科院分区：
文献类型：
--
作者：
Dillon, R. J.;Webster, G.;Charnley, A. K.
通过对4种野生蝗虫肠道细菌16S rRNA基因片段的变性梯度凝胶电泳(DGGE)分析，确定了其肠道细菌群落。这项研究揭示了阶段多态对来自南非的棕色蝗虫肠道细菌多样性的影响。一个单一的细菌系统型，与柠檬酸杆菌一致。在西班牙的摩洛哥和意大利蝗虫的两个同域种群的肠道微生物区系中占据主导地位。有证据表明有沃尔巴克氏菌。在英国当地捕获的草甸蝗虫。对DGGE产物的序列分析没有发现不可培养细菌的证据，同源性表明细菌物种主要是肠杆菌科的革兰氏杆菌，类似于以前在实验室饲养的蝗虫中记录的细菌。(C)2007 Elsevier Inc.保留所有权利。
The gut bacterial community from four species of feral locusts and grasshoppers was determined by denaturing gradient gel electrophoresis (DGGE) analysis of bacterial 16S rRNA gene fragments. The study revealed an effect of phase polymorphism on gut bacterial diversity in brown locusts from South Africa. A single bacterial phylotype, consistent with Citrobacter sp. dominated the gut microbiota of two sympatric populations of Moroccan and Italian locusts in Spain. There was evidence for Wollbachia sp. in the meadow grasshopper caught locally in the UK. Sequence analysis of DGGE products did not reveal evidence for unculturable bacteria and homologies suggested that bacterial species were principally Gammapoteobacteria from the family Enterobacteriaceae similar to those recorded previously in laboratory reared locusts. (C) 2007 Elsevier Inc. All rights reserved.