Functionally deficient neuronal differentiation of mouse embryonic neural stem cells in vitro
Functionally deficient neuronal differentiation of mouse embryonic neural stem cells in vitro
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DOI：
10.1016/j.neures.2004.02.010
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发表时间：
2004-06-01
影响因子：
2.9
通讯作者：
Copray, JM
中科院分区：
文献类型：
--
作者：
Balasubramaniyan, V;de Haas, AH;Copray, JM
从E14小鼠中分离胚胎小鼠神经干细胞（NSCs），在含有表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的培养基中增殖，并接种在无血清的基本神经基础培养基中的层粘连蛋白包被的威尔斯孔中。在体外培养7天后，大约20%的胚胎小鼠神经干细胞发育成形态和生化完全成熟的神经元，具有广泛的树突和多个突触接触。然而，即使在培养22天后，这些神经元中也没有一个发育出功能神经元特有的电压依赖性钠通道。显然，胚胎小鼠NSC向神经元的形态分化和电生理成熟是独立调节的。(C)2004年爱思唯尔爱尔兰有限公司和日本神经科学学会。All rights reserved.
Embryonic mouse neural stem cells (NSCs) were isolated from E14 mice, multiplied in medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and plated in laminin-coated wells in basic serum-free neurobasal medium. After 7 days in vitro, approximately 20% of the embryonic mouse NSCs developed into morphologically and biochemically fully maturated neurons, with extensive dendrites and multiple synaptic contacts. However, even after 22 days of culture, none of these neurons developed voltage-dependent sodium-channels characteristic for a functional neuron. Apparently, the morphological differentiation and the electrophysiological maturation of an embryonic mouse NSC into a neuron are independently regulated. (C) 2004 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.