The molecular characterization of the brain protein 44-like (Brp44l) gene of Gekko japonicus and its expression changes in spinal cord after tail amputation
The molecular characterization of the brain protein 44-like (Brp44l) gene of Gekko japonicus and its expression changes in spinal cord after tail amputation
复制标题
日本壁虎脑蛋白44样(Brp44l)基因的分子特征及其断尾后脊髓中的表达变化
DOI：
10.1007/s11033-007-9169-0
复制
发表时间：
2009-02-01
影响因子：
2.8
通讯作者：
Liu, Yan
中科院分区：
文献类型：
--
作者：
Jiang, Maorong;Gu, Xiaosong;Liu, Yan
从日本壁虎脑和脊髓cDNA文库中鉴定出编码脑蛋白44-like （Brp44l）的cDNA。它包含一个324bp的开放阅读框，对应于107个氨基酸的推断蛋白。BRP44L蛋白分子量为12.2664 kDa，理论pI为9.67。在氨基酸水平上，壁虎BRP44L与其他物种的相似性为85-89%。Northern blotting结果显示，Brp44l在日本壁虎的脑、脊髓、心、肺、肝、肾等主要器官中普遍表达，提示该基因具有重要的生物学作用。原位杂交（ISH）显示脊髓灰质中存在阳性杂交信号。采用逆转录聚合酶链反应（RT-PCR）检测断尾后脊髓组织中Brp44l的表达变化。该基因在断尾后脊髓中的表达水平升高，在断尾后1周达到最高值，说明Brp44l的表达水平可能与脊髓损伤和再生过程有关。
The cDNA encoding brain protein 44-like (Brp44l) was identified from the brain and spinal cord cDNA library of Gekko japonicus. It contains a 324 bp open reading frame corresponding to a deduced protein of 107 amino acids. The molecular weight of the BRP44L protein is 12.2664 kDa and its theoretical pI is 9.67. At amino acid level, the BRP44L from gecko shares 85–89% similarity with those from other species. Northern blotting showed that Brp44l was ubiquitously expressed in the major organs of Gekko japonicus including brain, spinal cord, heart, lung, liver, and kidney, suggesting a fundamental biological role of this gene. In situ hybridization (ISH) revealed that positive hybridization signals were present in gray matter of spinal cord. The change of Brp44l expression in spinal cord after tail amputation was examined by reverse transcription polymerase chain reaction (RT-PCR). The expression level of this gene increased in the spinal cord after tail amputation, and reached the highest value at 1 week after tail amputation, which indicated that the expression level of Brp44l might be associated with the process of spinal cord injury and regeneration.