Selection of nuclear genotypes associated with the thermo-sensitivity of Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.)
Selection of nuclear genotypes associated with the thermo-sensitivity of Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.)
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DOI：
10.1007/s00122-022-04046-7
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发表时间：
2022-02-11
影响因子：
5.4
通讯作者：
Kubo，Tomohiko
中科院分区：
文献类型：
--
作者：
Matsuhira，Hiroaki;Kitazaki，Kazuyoshi;Kubo，Tomohiko
当与特定基因型结合时，甜菜细胞质雄性不育变得对温度敏感，这可能为利用该性状进行环境控制授粉提供了一种手段。摘要研究了甜菜（Beta vulgarisL.）在不同遗传背景下细胞质雄性不育表达的稳定性。将9株开放授粉品种的遗传异质性植株与细胞质雄性不育系杂交，得到266株f1植株。利用与育性恢复1（Rf1）连锁的多等位基因DNA标记进行标记分析，将f1植株分为15个基因型。我们在开花季节有白天加热和没有白天加热的温室房间两种环境条件下评估了f1植株的表型。出现了三个表型组：一致表达雄性不育的表型组、一致恢复花粉育性的表型组和以热敏方式表达雄性不育的表型组。在一贯雄性不育组中，所有植株都遗传了一种名为p4的特定rf1标记类型。本研究对温敏雄性不育植物作为杂交种子亲本的潜力进行了测试，选择了3种基因型。具有红色下胚轴和叶脉优势性状的花粉亲本系在温敏雄性不育植株上进行开放授粉，结果表明其雌性器官功能正常。99.9%以上的后代表达红色色素沉着性状；因此，获得了高纯度的杂交种。我们从三个基因型中确定了rf1的核苷酸序列：一个有一个新的等位基因，两个有已知的等位基因，其中一个据报道在美国的一个育种站被选为非恢复等位基因，但在美国、欧洲或日本的其他育种站没有，这表明环境敏感性。
Key messageCytoplasmic male sterility in sugar beet becomes thermo-sensitive when combined with specific genotypes, potentially offering a means to environmentally control pollination by this trait.AbstractThe stability of cytoplasmic male sterility expression in several genetic backgrounds was investigated in sugar beet (Beta vulgarisL.). Nine genetically heterogenous plants from open-pollinated varieties were crossed with a cytoplasmic male sterile line to obtain 266 F1plants. Based on marker analysis using a multiallelic DNA marker linked torestorer-of-fertility 1(Rf1), we divided the F1plants into 15 genotypes. We evaluated the phenotypes of the F1plants under two environmental conditions: greenhouse rooms with or without daytime heating during the flowering season. Three phenotypic groups appeared: those consistently expressing male sterility, those consistently having restored pollen fertility, and those expressing male sterility in a thermo-sensitive manner. All plants in the consistently male sterile group inherited a specificRf1marker type named p4. We tested the potential for thermo-sensitive male sterile plants to serve as seed parents for hybrid seed production, and three genotypes were selected. Open pollination by a pollen parental line with a dominant trait of red-pigmented hypocotyls and leaf veins resulted in seed setting on thermo-sensitive male sterile plants, indicating that their female organs were functional. More than 99.9% of the progeny expressed the red pigmentation trait; hence, highly pure hybrids were obtained. We determined the nucleotide sequences ofRf1from the three genotypes: One had a novel allele and two had known alleles, of which one was reported to have been selected previously as a non-restoring allele at a single U.S. breeding station but not at other stations in the U.S., or in Europe or Japan, suggesting environmental sensitivity.