Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa.
Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa.
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DOI：
10.1128/aac.00878-13
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发表时间：
2013-09-01
影响因子：
4.9
通讯作者：
Melano, Roberto G
中科院分区：
文献类型：
--
作者：
Tijet, Nathalie;Boyd, David;Melano, Roberto G
对244株产碳青霉烯酶和不产碳青霉烯酶的肠杆菌科细菌和铜绿假单胞菌进行了Carba NP检测。我们证实了该试验100%的特异度和阳性预测值，但敏感度和阴性预测值分别为72.5%和69.2%，而使用浓度更高的细菌提取物，敏感度和阴性预测值分别增加到80%和77.3%。假阴性结果与粘液菌株有关，或与碳青霉烯酶活性低的酶有关，特别是在肠道细菌中普遍出现的类似OXA-48的酶。
The Carba NP test was evaluated against a panel of 244 carbapenemase- and non-carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa isolates. We confirmed the 100% specificity and positive predictive value of the test, but the sensitivity and negative predictive value were 72.5% and 69.2%, respectively, and increased to 80% and 77.3%, respectively, using a more concentrated bacterial extract. False-negative results were associated with mucoid strains or linked to enzymes with low carbapenemase activity, particularly OXA-48-like, which has emerged globally in enterobacteria.