Nerve growth factor concentrations in the synovial fluid from healthy dogs and dogs with secondary osteoarthritis
Nerve growth factor concentrations in the synovial fluid from healthy dogs and dogs with secondary osteoarthritis
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DOI：
10.3415/vcot-10-04-0051
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发表时间：
2011-01-01
影响因子：
1.3
通讯作者：
Busetto, R.
中科院分区：
文献类型：
--
作者：
Isola, M.;Ferrari, V.;Busetto, R.
目的：测定正常犬和继发性骨关节炎(OA)犬关节滑液中神经生长因子(NGF)的浓度。方法：采用双抗体夹心酶联免疫吸附试验(EL ISA)测定50只健康犬(12只)、16只急性跛行犬(16只)和22只慢性跛行患者(22只)的滑液中神经生长因子(NGF)浓度。急性和慢性跛行都是继发于涉及肩、肘和窒息关节的骨科疾病。神经生长因子滑膜浓度在健康组和急性组的平均值之间以及三组之间的比较使用F检验。结果：所有犬关节液标本均检测到神经生长因子。而正常组滑膜NGF浓度(3.65±2.18pg/ml)与急性跛行组(6.45+/-2.45pg/ml)比较差异无统计学意义(p=0.79)。相反，慢性跛行犬关节滑液中NGF的平均浓度(20.19+/-17.51 pg/ml)显著高于健康犬(p<；0.01)。临床意义：本研究首次证实慢性跛行犬关节液中NGF的存在及其浓度高于健康犬和急性跛行犬。慢性跛行和滑膜浓度升高之间的关联可能表明NGF参与了骨性关节炎炎症和慢性疼痛。
Objective: To measure the concentrations of nerve growth factor (NGF) in the synovial fluid from normal dogs and dogs with osteoarthritis (OA) secondary to common joint disorders.Methods: Nerve growth factor synovial concentrations were measured by ELISA assay in 50 dogs divided into three groups; 12 healthy, 16 affected by acute lameness within seven days before enrolment, and 22 with chronic lameness persisting by more than one month before enrolment and accompanied by radiological signs of OA. Both acute and chronic lameness were secondary to orthopaedic diseases involving the shoulder, elbow and stifle joints. Nerve growth factor synovial concentrations were compared between means for healthy and acute groups and between thethree groups using an F-test. Significance level was set at p < 0.05.Results: Nerve growth factor was detected in all canine synovial fluid samples. However, the mean synovial NGF concentration of healthy dogs (3.65 +/- 2.18 pg/ml) was not significantly different from the mean value in dogs with acute lameness (6.45 +/- 2.45 pg/ml) (p =0.79). Conversely, the mean synovial NGF concentration in dogs with chronic lameness (20.19 +/- 17.51 pg/ml) was found to be significantly higher than that found in healthy dogs (p < 0.01).Clinical significance: This study demonstrates for the first time the presence of NGF in canine synovial fluid and its increased concentrations in dogs with chronic lameness compared to healthy dogs and dogs with acute lameness. The association between chronic lameness and raised synovial concentrations may suggest an involvement of NGF in OA inflammation and chronic pain.