Plasma IP-10 Is Increased in Immunological NonResponders and Associated With Activated Regulatory T Cells and Persisting Low CD4 Counts
Plasma IP-10 Is Increased in Immunological NonResponders and Associated With Activated Regulatory T Cells and Persisting Low CD4 Counts
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DOI：
10.1097/qai.0000000000001080
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发表时间：
2016-10-01
影响因子：
3.6
通讯作者：
Dyrhol-Riise, Anne M.
中科院分区：
文献类型：
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作者：
Stiksrud, Birgitte;Lorvik, Kristina Berg;Dyrhol-Riise, Anne M.
目的：探索免疫机制并确定与接受有效抗逆转录病毒治疗的 HIV 患者免疫恢复不充分相关的生物标志物。设计：对 67 名 HIV 感染患者进行抗逆转录病毒治疗≥24 个月（HIV RNA 600 个细胞/微升）的横断面研究。纳入后 2 年也记录了 CD4 计数。方法：通过多重测定、ELISA 或质谱法测量血浆中的细胞因子、微生物易位的可溶性标记物和色氨酸分解代谢物。通过流式细胞术分析具有可比最低 CD4 计数的 2 个亚组中的 T 细胞活化、分化和调节性 T 细胞 (Treg)。结果：血浆干扰素诱导蛋白 10 (IP-10) 水平较高 (P < 0.05)，T 细胞更加活化 (CD38(+)HLA-DR+) (P < 0.05)，初始/效应记忆 T 细胞比率较低与免疫应答者相比，INR 患者的静息 Tregs 比例（CD4(+)CD45RA(+)FoxP3(+)）降低（P < 0.01）（P < 0.001）。 INR 患者的 CD4 计数
Objective: To explore immune mechanisms and identify biomarkers associated with an inadequate immune recovery in patients with HIV with efficient antiretroviral therapy.Design: A cross-sectional study of 67 HIV-infected patients on antiretroviral therapy for >= 24 months with HIV RNA 600 cells per microliter). CD4 counts were also registered 2 years after inclusion.Methods: Cytokines, soluble markers of microbial translocation, and tryptophan catabolites were measured in plasma by multiplex assay, ELISA, or mass spectrometry. T-cell activation, differentiation, and regulatory T cells (Tregs) were analyzed by flow cytometry in 2 subgroups with comparable nadir CD4 counts.Results: Plasma interferon-inducible protein-10 (IP-10) levels were higher (P < 0.05), the T cells were more activated (CD38(+)HLA-DR+) (P < 0.05), the naive/effector memory T-cell ratio was lower (P < 0.01) and the proportion of resting Tregs (CD4(+)CD45RA(+)FoxP3(+)) was reduced (P < 0.001) in INR patients compared with immunological responders. INR patients with CD4 counts