VENTRAL EXPRESSION OF GATA-1 AND GATA-2 IN THE XENOPUS EMBRYO DEFINES INDUCTION OF HEMATOPOIETIC MESODERM
VENTRAL EXPRESSION OF GATA-1 AND GATA-2 IN THE XENOPUS EMBRYO DEFINES INDUCTION OF HEMATOPOIETIC MESODERM
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DOI：
10.1006/dbio.1994.1246
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发表时间：
1994-09-01
影响因子：
2.7
通讯作者：
ZON, LI
中科院分区：
文献类型：
--
作者：
KELLEY, C;YEE, K;ZON, LI
由于缺乏分子标记物，参与中胚层定型形成血液的早期胚胎事件尚未被详细研究。我们研究了造血转录因子加塔-1和加塔-2在非洲爪蟾胚胎发生过程中的表达。在发育过程中，加塔-1的表达定位于胚胎的腹侧区域，并且先于胚胎球蛋白的表达。加塔-2在原肠胚形成结束时在胚胎的腹侧区域高度表达，随后在血岛区域和中枢神经系统中表达。锂诱导的背侧化的胚胎废除加塔-2的表达，和紫外线诱导的腹侧化的胚胎导致径向对称的表达加塔-2。因此，加塔-2的表达反映了胚胎的腹侧特征。GATA结合蛋白和珠蛋白在腹侧边缘区外植体的表达表明，造血早在囊胚阶段就被编程。加塔-1和加塔-2也在培养的动物帽外植体中表达，表明这些细胞具有造血潜能。加塔-1和加塔-2的发育表达与它们在高等生物体中的造血作用一致，并且限定了产生造血祖细胞的早期胚胎的腹侧区域。我们的研究表明，这些基因将是有用的，在定义的诱导事件，导致造血中胚层的形成。(C)1994年出版社出版。
The early embryonic events involved in the commitment of mesoderm to form blood have not been studied in detail for lack of molecular markers. We have studied the expression of the hematopoietic transcription factors GATA-1 and GATA-2 during Xenopus embryogenesis. During development GATA-1 expression is localized to the ventral region of the embryo and precedes the expression of embryonic globins. GATA-2 is highly expressed in the ventral region of the embryo by the end of gastrulation and later is expressed in the blood island region and the central nervous system. Lithium-induced dorsalization of embryos abrogates GATA-2 expression, and uv-induced ventralization of embryos leads to a radially symmetrical expression of GATA-2. Therefore, GATA-2 expression reflects the ventral character of the embryo. The expression of the GATA-binding proteins and globin in ventral marginal zone explants demonstrates that hematopoiesis is programmed as early as the blastula stage. GATA-1 and GATA-2 are also expressed in cultured animal cap explants, suggesting that these cells have hematopoietic potential. The developmental expression of GATA-1 and GATA-2 is consistent with their role in hematopoiesis in higher organisms and defines the ventral regions of the early embryo that give rise to hematopoietic progenitors. Our studies indicate that these genes will be useful in defining the inductive events that lead to the formation of hematopoietic mesoderm. (C) 1994 Academic Press, Inc.