STRESS-RESPONSE, ADRENAL-STEROID RECEPTOR LEVELS AND CORTICOSTEROID-BINDING GLOBULIN LEVELS - A COMPARISON BETWEEN SPRAGUE-DAWLEY, FISCHER-344 AND LEWIS RATS
STRESS-RESPONSE, ADRENAL-STEROID RECEPTOR LEVELS AND CORTICOSTEROID-BINDING GLOBULIN LEVELS - A COMPARISON BETWEEN SPRAGUE-DAWLEY, FISCHER-344 AND LEWIS RATS
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DOI：
10.1016/0006-8993(93)90196-t
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发表时间：
1993-07-09
期刊：
影响因子：
2.9
通讯作者：
SPENCER, RL
中科院分区：
文献类型：
--
作者：
DHABHAR, FS;MCEWEN, BS;SPENCER, RL
组织相容性 Fischer 344 (F344) 和 Lewis (LEW) 大鼠为研究神经、内分泌和免疫系统之间的相互作用提供了比较模型。近交系 Sprague-Dawley (SD) 是近交系 F344 和 LEW 品系的母本品系。在本研究中，我们报告了这三种遗传相关品系的昼间和应激皮质酮（CORT）谱存在巨大差异：（1）F344 大鼠的昼间和应激皮质酮水平显着高于 SD 和 LEW 大鼠； (2)早晨，应激源停止后1 h，SD和F344大鼠的应激CORT水平恢复至基础水平，而此时LEW大鼠的应激CORT水平尚未恢复至基础水平； (3)在晚上，SD和F344大鼠的基础CORT水平表现出预期的夜间上升，而LEW大鼠则没有表现出这种上升。鉴于 CORT 水平的巨大差异，我们期望观察神经组织和免疫组织中 I 型（盐皮质激素）和 II 型（糖皮质激素）肾上腺类固醇受体绝对水平的应变差异。然而，我们发现海马、下丘脑、垂体、胸腺、脾脏和外周血单核细胞中 I 型受体水平没有显着的菌株差异。同样，我们发现大多数调查组织中 II 型受体水平没有显着的应变差异，但值得注意的例外是 LEW 大鼠在胸腺中显示出较高的 II 型结合，而 SD 大鼠在海马中显示出较小但显着较高的 II 型结合。我们还研究了皮质类固醇结合球蛋白（CBG）水平的菌株差异。 F344 大鼠在血浆、脾脏和胸腺中表达的 CBG 水平显着高于 SD 和 LEW 大鼠。未来的研究将探讨在肾上腺切除后神经和免疫组织中肾上腺类固醇受体水平几乎没有菌株差异的情况下，菌株之间 CORT 和 CBG 水平的显着差异是否会转化为静息条件下或应激后受体占用/激活的差异。
Histocompatible Fischer 344 (F344) and Lewis (LEW) rats provide a comparative model for investigating the interactions between the nervous, endocrine and immune systems. The outbred Sprague-Dawley (SD) is the maternal strain for the inbred F344 and LEW strains. In this study we report large differences in the diurnal and stress corticosterone (CORT) profiles of these three genetically related strains: (1) F344 rats had significantly higher diurnal and stress CORT levels than SD and LEW rats; (2) in the morning, stress CORT levels of SD and F344 rats returned towards basal 1 h after cessation of the stressor, whereas stress CORT levels of LEW rats had not returned to basal by this time; and (3) in the evening, SD and F344 rats showed the expected evening rise in basal CORT levels, whereas LEW rats failed to show this rise. In light of the large differences in CORT levels, we expected to observe strain differences in absolute levels of Type I (mineralocorticoid) and Type II (glucocorticoid) adrenal steroid receptors in neural as well as immune tissue. However, we found no significant strain differences in levels of Type I receptors in the hippocampus, hypothalamus, pituitary, thymus, spleen and peripheral blood mononuclear cells. Similarly, we saw no significant strain differences in levels of Type II receptors in most of the tissues surveyed, with the notable exception that LEW rats showed higher Type II binding in the thymus, and SD rats showed small, but significantly higher Type II binding in the hippocampus. We also studied strain differences in levels of corticosteroid-binding globulin (CBG). F344 rats expressed significantly higher CBG levels than SD and LEW rats, in plasma, spleen and thymus. Future studies will investigate whether the substantial differences between strains in levels of CORT and CBG, in the context of few strain differences in post-adrenalectomy adrenal steroid receptor levels in neural and immune tissue, translate into differences in receptor occupancy/activation under resting conditions, or following stress.