A composite coating by electrolysis-induced collagen self-assembly and calcium phosphate mineralization
A composite coating by electrolysis-induced collagen self-assembly and calcium phosphate mineralization
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DOI：
10.1016/j.biomaterials.2004.06.019
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Wang, RZ
中科院分区：
文献类型：
--
作者：
Fan, YW;Duan, K;Wang, RZ
由胶原蛋白和磷酸钙矿物质组成的复合涂层被认为具有生物活性，可以促进骨生长和金属骨科植入物的固定。本研究我们成功地通过电解沉积（ELD）在硅基底上开发了均匀的胶原纤维/磷酸八钙复合涂层。涂层沉积是通过在含有胶原分子的弱酸性（pH 4.8-5.3）水溶液的三电极电化学池中向阴极施加恒定电势来完成的。钙和磷酸根离子。涂层过程涉及胶原纤维的自组装和由于阴极反应和局部pH增加而导致的磷酸钙矿物的沉积。通过适当调节溶液和沉积参数，这两个步骤可以同步进行，形成纳米尺度的类骨复合材料。通过光学显微镜和荧光显微镜分析了涂层的形貌、晶体结构和成分。扫描和透射电子显微镜，能量色散X射线分析。电感耦合氩等离子体光发射分光光度法和傅立叶变换红外光谱法。在典型的沉积条件下，阴极（Si）表面形成磷酸钙涂层的薄（100 nm）层，在其顶部形成厚（类似于100 μ m）复合层。多孔复合材料层由胶原纤维网络组成，磷酸八钙晶体簇在胶原纤维网络上成核和生长。通过结合光刻和ELD。我们还能够将复合涂层图案化为规则的正方形阵列。纳米压痕测试的初步结果表明，适当制备的复合涂层可能比整体多孔磷酸钙涂层具有更高的弹性模量和抗划伤性。研究结果不仅为生物医用植入物提供了一种新型的生物活性涂层，而且为研究胶原基生物组织的生物矿化机理建立了一种新的实验方法。(C)2004爱思唯尔有限公司保留所有权利。
A composite coating that is composed of collagen protein and calcium phosphate minerals is considered to be bioactive and may enhance bone growth and fixation of metallic orthopedic implants. In this study. we have successfully developed a uniform collagen fibril/octacalcium phosphate composite coating on silicon substrate by electrolytic deposition (ELD). The coating deposition %vas done through applying a constant potential to the cathode in a three-electrode electrochemistry cell that contain a mild acidic (pH 4.8-5.3) aqueous solution of collagen molecules. calcium and phosphate ions. The coating process involved self-assembly of collagen fibrils and the deposition of calcium phosphate minerals as a result of cathode reaction and local pH increase. The two steps could be synchronized to form a bone-like composite at nanometer scale through proper adjustment of the solution and deposition parameters. Coating morphology, crystal structure and compositions were analyzed by optical and fluorescence microscopy. scanning and transmission electron microscopy, energy dispersive X-ray analysis. inductively coupled argon plasma optical emission spectrophotometry, and Fourier-transformed infrared spectroscopy. Under typical deposition conditions, the cathode (Si) surface formed a thin (100 nm) layer of calcium phosphate coating, on top of which a thick (similar to 100 mum) composite layer formed. The porous composite layer consists of a collagen fibril network on which clusters of octacalcium phosphate crystals nucleate and grow. By combining photolithography and ELD. we were also able to pattern the composite coating into regular arrays of squares. Preliminary results by nanoindentation tests showed that properly prepared composite coating may have higher elastic modulus and scratch resistance than monolithic porous calcium phosphate coating. The results not only provide a novel bioactive coating for biomedical implants, but also establish a new experimental protocol for studying biomineralization mechanisms of collagen based biological tissues. (C) 2004 Elsevier Ltd. All rights reserved.