TPM2 as a potential predictive biomarker for atherosclerosis
TPM2 as a potential predictive biomarker for atherosclerosis
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DOI：
10.18632/aging.102231
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发表时间：
2019-09-15
期刊：
影响因子：
5.2
通讯作者：
Gong, Tao
中科院分区：
文献类型：
--
作者：
Meng, Ling-Bing;Shan, Meng-Jie;Gong, Tao
心脑血管疾病（CCVD）主要由动脉粥样硬化引起，是导致死亡的主要原因。许多研究试图阐明动脉粥样硬化的分子机制，但其发病机制尚未完全阐明。两个表达谱数据集，GSE 43292和GSE 57691，从基因表达综合数据库（GEO）获得。本研究确定了差异表达基因（DEG 5），并进行功能注释。最后，构建动脉粥样硬化动物模型和神经网络预测模型，验证hub基因与动脉粥样硬化的关系。结果在正常和动脉粥样硬化样品之间鉴定了总共234个DEG 5。DEG 5主要富集在肌动蛋白丝、肌动蛋白结合、平滑肌细胞和精氨酸-细胞因子受体相互作用中。共鉴定出13个基因为枢纽基因。经动物模型验证，发现了常见的DEG--原肌球蛋白2（Tropomyosin 2，TPM 2），其在动脉粥样硬化模型和样品中以较低水平显示。综上所述，DEG 5在本研究中的鉴定可能有助于临床医生了解动脉粥样硬化的发生和发展的发病机制，而TPM 2显示出作为动脉粥样硬化的有希望的诊断和治疗生物标志物的潜力。
Cardiac-cerebral vascular disease (CCVD), is primarily induced by atherosclerosis, and is a leading cause of mortality. Numerous studies have investigated and attempted to clarify the molecular mechanisms of atherosclerosis; however, its pathogenesis has yet to be completely elucidated. Two expression profiling datasets, GSE43292 and GSE57691, were obtained from the Gene Expression Omnibus (GEO) database. The present study then identified the differentially expressed genes (DEG5), and functional annotation of the DEG5 was performed. Finally, an atherosclerosis animal model and neural network prediction model was constructed to verify the relationship between hub gene and atherosclerosis. The results identified a total of 234 DEG5 between the normal and atherosclerosis samples. The DEG5 were mainly enriched in actin filament, actin binding, smooth muscle cells, and cytokine-cytokine receptor interactions. A total of 13 genes were identified as hub genes. Following verification of animal model, the common DEG, Tropomyosin 2 (TPM2), was found, which were displayed at lower levels in the atherosclerosis models and samples. In summary, DEG5 identified in the present study may assist clinicians in understanding the pathogenesis governing the occurrence and development of atherosclerosis, and TPM2 exhibits potential as a promising diagnostic and therapeutic biomarker for atherosclerosis.