Immunohistochemical expression of mucin antigens in gallbladder adenocarcinoma: MUC1-positive and MUC2-negative expression is associated with vessel invasion and shortened survival
Immunohistochemical expression of mucin antigens in gallbladder adenocarcinoma: MUC1-positive and MUC2-negative expression is associated with vessel invasion and shortened survival
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DOI：
10.14670/hh-11-824
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发表时间：
2017-06-01
影响因子：
2
通讯作者：
Tanimoto, Akihide
中科院分区：
文献类型：
--
作者：
Hiraki, Tsubasa;Yamada, Sohsuke;Tanimoto, Akihide
粘蛋白在影响癌症生物学方面发挥着关键作用，例如影响癌症侵袭、侵袭性和/或转移潜力。我们的目的是研究两种粘蛋白（特别是 MUC1 和 MUC2）表达谱的意义、它们与胆囊腺癌 (GBAC) 各种临床病理特征和预后的相关性。我们使用抗粘蛋白核心蛋白 MUC1/DF3 和 MUC2/Ccp58 的抗体对 81 个石蜡包埋的肿瘤样本进行了手术切除 GBAC 患者的免疫组织化学分析。当≥20%或10%的GBAC细胞分别显示阳性染色时，MUC1或MUC2表达被认为是高的。研究表明，MUC1 高表达与病理性淋巴管和血管侵犯以及区域淋巴结转移有显着关系。相比之下，MUC2 高表达与病理性神经周围浸润、T 分期≥ 3 和术后复发显着相关。此外，MUC1 与 MUC2 表现出显着的正共表达和潜在的互补相关性。多变量分析表明，MUC1 高表达组的疾病特异性生存时间显着缩短。然而，MUC1 和 MUC2 高表达的组合并不能预测 GBAC 的结果会更差。因此，虽然每种粘蛋白在GBAC进展的发病机制中都具有一定的重要作用，但MUC1可以独立预测GBAC患者的血管侵犯和不良预后。 MUC1 的检测很可能为针对术后 GBAC 提供临床管理和治疗提供有用的参数。
Mucins play pivotal roles in influencing cancer biology, for example affecting carcinoma invasion, aggressiveness and/or metastatic potential. Our aim is to investigate the significance of expression profiles of two mucins in particular, MUC1 and MUC2, their correlations with various clinicopathological features, and prognosis in gallbladder adenocarcinoma (GBAC). We performed immunohistochemistry from patients with surgically resected GBAC, using antibodies against mucin core proteins MUC1/DF3 and MUC2/Ccp58 in 81 paraffin-embedded tumor samples. MUC1 or MUC2 expression was considered to be high when >= 20% or 10% of the GBAC cells showed positive staining, respectively. High MUC1 expression was revealed to have a significant relationship to the presence of pathologically lymphatic and vascular invasion, and regional lymph node metastasis. By contrast, high MUC2 expression showed a significant correlation with pathologically perineural invasion, T stage >= 3, and post-operative recurrence. Moreover, MUC1 showed significantly positive co-expression and potentially complementary correlations with MUC2. Multivariate analyses demonstrated that the high MUC1 expression group had significantly shorter disease-specific survival times. However, the combination of both high MUC1 and MUC2 expression did not predict worse outcome in GBACs. Therefore, although each mucin has a somewhat important role in the pathogenesis of GBAC progression, MUC1 can independently predict vessel invasion and poor prognosis in patients with GBAC. The detection of MUC1 might well offer a useful parameter for providing clinical management and treatment against postsurgical GBACs.