Fluorine-19 nuclear magnetic resonance studies of lipid fatty acyl chain order and dynamics in Acholeplasma laidlawii B membranes. A physical, biochemical, and biological evaluation of monofluoropalmitic acids as membrane probes.
Fluorine-19 nuclear magnetic resonance studies of lipid fatty acyl chain order and dynamics in Acholeplasma laidlawii B membranes. A physical, biochemical, and biological evaluation of monofluoropalmitic acids as membrane probes.
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无胆原体莱氏 B 膜中脂质脂肪酰基链顺序和动力学的氟 19 核磁共振研究。
DOI：
10.1021/bi00291a008
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
R. McElhaney
中科院分区：
文献类型：
--
作者：
B. McDonough;P. Macdonald;B. Sykes;R. McElhaney
氟-19核磁共振光谱为研究模型和生物膜中的脂质脂肪酰基链顺序和动力学提供了许多独特的优势。然而，常用作19 F膜探针的香叶醇二氟亚甲基脂肪酸似乎明显干扰模型膜和生物膜的组织。因此，我们已经合成了一系列的特异性标记的monofluoropalmitic酸和评估这些作为合适的膜探针。差示扫描量热法研究几个双-（monofluoropalmitoyl）磷脂酰胆碱的水分散体显示，棕榈酸的羰基附近的氟取代只有适度的影响，这些模型膜的热致相行为，在中心或朝向甲基末端的取代是相对nonperturbing。此外，所有测试的双（单氟棕榈酰）磷脂酰胆碱在所有比例下与二棕榈酰磷脂酰胆碱表现出几乎理想的混合。简单的无细胞壁原核生物莱氏无胆甾原体B的膜的热致相行为也没有被可检测的生物合成掺入的单氟棕榈酸的量的存在下改变。我们还发现，即使是大量的单氟棕榈酸的生物合成纳入到膜脂的A。Laidlawii B对该生物体的生长和存活没有影响。生长培养基中外源性单氟棕榈酸的存在不会改变A. Laidlawii B膜此外，所有测试的单氟棕榈酸以及棕榈酸本身都是生物合成的，并且在各种膜糖脂和磷脂之间相对均匀地分布。（250字处删节）
Fluorine-19 nuclear magnetic resonance spectroscopy offers a number of unique advantages for studies of lipid fatty acyl chain order and dynamics in model and biological membranes. However, the germinal difluoromethylene fatty acids commonly employed as 19F membrane probes appear to appreciably perturb the organization of model membranes and biomembranes. We have thus synthesized a series of specifically labeled monofluoropalmitic acids and evaluated these as suitable membrane probes. Differential scanning calorimetric studies of aqueous dispersions of several bis-(monofluoropalmitoyl)phosphatidylcholines reveal that a fluorine substitution near the carbonyl group of palmitic acid has only a modest effect on the thermotropic phase behavior of these model membranes and that substitutions in the center or toward the methyl terminus are relatively nonperturbing. Moreover, all bis(monofluoropalmitoyl)phosphatidylcholines tested exhibit nearly ideal mixing in all proportions with dipalmitoylphosphatidylcholine. The thermotropic phase behavior of membranes of the simple, cell-wall-less prokaryote Acholeplasma laidlawii B is also not detectably altered by the presence of appreciable amounts of biosynthetically incorporated monofluoropalmitic acid. We also find that the biosynthetic incorporation of even large amounts of monofluoropalmitic acids into the membrane lipids of A. laidlawii B has no effect upon the growth and survival of this organism. The presence of exogenous monofluoropalmitic acids in the growth medium does not alter the polar head group composition or lipid/protein ratio of the A. laidlawii B membrane. In addition, all monofluoropalmitic acids tested are biosynthetically incorporated as well as palmitic acid itself and distribute relatively evenly between the various membrane glyco- and phospholipids.(ABSTRACT TRUNCATED AT 250 WORDS)
特定氘代氟自旋探针的氘核磁共振。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Oldfield,E;Lee,RW;Meadows,M;Dowd,SR;Ho,C
通讯作者：
Ho,C
大肠杆菌膜中蛋白质-脂质相互作用的生物物理学研究。
影响因子：
3.4
作者：
Gent,MP;Cottam,PF;Ho,C
通讯作者：
Ho,C
影响因子：
2.9
作者：
Chen,SC;Sturtevant,JM;Conklin,K;Gaffney,BJ
通讯作者：
Gaffney,BJ