Core-shell mesoporous silica nanoparticles improve HeLa cell growth and proliferation inhibition by (-)-epigallocatechin-3-gallate by prolonging the half-life
Core-shell mesoporous silica nanoparticles improve HeLa cell growth and proliferation inhibition by (-)-epigallocatechin-3-gallate by prolonging the half-life
复制标题
核壳介孔二氧化硅纳米粒子通过延长半衰期改善 (-)-表没食子儿茶素-3-没食子酸酯对 HeLa 细胞生长和增殖的抑制
DOI：
10.1039/c2jm32271d
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
He, Zhiwei
中科院分区：
文献类型：
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作者：
Ding, Jie;Kong, Xia;He, Zhiwei
（-）-表没食子儿茶素-3-没食子酸酯（EGCG）是一种对多种癌细胞系有效的抗癌药物，但在不同的细胞凋亡培养条件下，其半衰期为30分钟至2小时，不稳定;形成EGCG二聚体、过氧化氢（H2 O2）和其他氧化产物。在本文中，我们报告了一种多核-壳功能化的胶体介孔二氧化硅纳米粒子系统（CMS），导致表没食子儿茶素没食子酸酯（EGCG）的表面和孔的静电吸引力，减轻产生二聚体或其他聚合物的自由基的相互作用。基于MTT测定、细胞周期分析和western blot的结果，与在没有CMS的情况下用于处理HeLa细胞的游离EGCG相比，将EGCG负载到CMS中增加了EGCG的抗癌能力。此外，异硫氰酸荧光素（FITC）被捕获在CMS的核心，这使得CMS可以同时指示EGCG在细胞中的位置。这些结果表明，有可能使用CMS平台作为促进剂来改善不稳定化疗剂的抗癌能力。
(-)-Epigallocatechin-3-gallate (EGCG) is an effective anticancer drug for a variety of cancer cell lines, but it is unstable with a half-life of 30 minutes to 2 hours under different culture conditions for cell apoptosis; EGCG dimers, hydrogen peroxide (H2O2) and other oxidative products are formed. Herein, we report that a multiple core-shell functionalized colloidal mesoporous silica nanoparticle system (CMS) resulted in the electrostatic attraction of EGCG to the surface and pores, alleviating the interaction of free radicals which produced dimers or other polymers. Based on the results of MTT assay, cell cycle analysis and western blot, the loading of EGCG into the CMS increased the anticancer ability of EGCG compared to that of free EGCG being used to treat HeLa cells in the absence of CMS. In addition, fluorescein isothiocyanate (FITC) was entrapped in the core of CMS, which allowed the CMS to simultaneously denote the position of EGCG in cells. These results demonstrate that it is possible to use the CMS platform as a promoter to improve the anticancer ability of the unstable chemotherapeutic agent.