Immunotoxicity assessment of ordered mesoporous carbon nanoparticles modified with PVP/PEG
Immunotoxicity assessment of ordered mesoporous carbon nanoparticles modified with PVP/PEG
复制标题
PVP/PEG修饰的有序介孔碳纳米粒子的免疫毒性评估
DOI：
10.1016/j.colsurfb.2018.07.072
复制
发表时间：
2018-11-01
影响因子：
5.8
通讯作者：
Yang, Feng
中科院分区：
文献类型：
--
作者：
Li, Xinfang;Wang, Linzhao;Yang, Feng
介孔碳纳米颗粒（MCN）具有较大的表面积和三维孔隙结构，作为潜在的药物载体引起了人们的极大兴趣。然而，MCN的免疫毒性至今尚未明确。本研究报道了在体外对树突状细胞（DCs）、T淋巴细胞和RAW264.7巨噬细胞等免疫细胞进行PVP或DSPE修饰和不修饰mPEG2000 （PEG）的MCN的影响。通过血液生化试验、alexin C3测定和组织学分析，探讨MCN的体内毒性。合成的MCN平均粒径约为90 nm，自然不溶于水。用PVP （MCN-PVP）或PEG （MCN-PEG）对介孔碳进行表面改性，使其粒径和Zeta电位略有增加，有效改善了介孔碳的分散性。MCN、MCN- pvp和MCN- peg促进了dc的分化和成熟，而MCN- pvp和MCN- peg显著抑制了分泌的tnf - α和IL-6水平。这些物质显著诱导T淋巴细胞凋亡。组织病理学结果显示，经过修饰或未经过修饰的纳米颗粒之间无显著差异。重要的是，在肺中观察到物质沉积，这可能会抑制肺转移。综上所述，PVP或PEG表面修饰的有序介孔碳纳米颗粒具有良好的免疫生物相容性，有望在制药和临床中作为药物载体。
With large surface area and three-dimensional pore structure, mesoporous carbon nanoparticles (MCN) have attracted enormous interests as potential drug carriers. However, MCN immunotoxicity has not been clarified clearly up to now. Herein we reported the effect of MCN with and without PVP or DSPE mPEG2000 (PEG) modification on immune cells including dendritic cells (DCs), T lymphocytes and RAW264.7 macrophages in vitro. Furthermore, blood biochemical tests, alexin C3 assay and histological analysis were used to investigate the toxicity of MCN in vivo. The synthesized MCN with average particle size about 90 nm was naturally insoluble in water. Surface modification with PVP (MCN-PVP) or PEG (MCN-PEG) slightly increased the particle size and Zeta potential, and effectively improved the dispersion of mesoporous carbon. MCN, MCN-PVP and MCN-PEG promoted the differentiation and maturation of the DCs, while the levels of secreted TNF-alpha and IL-6 were significantly suppressed by MCN-PVP and MCN-PEG. These materials significantly induced apoptosis of T lymphocytes. The histopathologic results showed that there was no significant difference between nanoparticles with or without modification. Importantly, the materials deposition was observed in the lung, which could potentially inhibit lung metastasis. In conclusion, the ordered mesoporous carbon nanoparticles superficially modified by PVP or PEG perform well in immunological biocompatibility, and are likely to be a promising candidate as medicine carrier in pharmaceutics and clinic.