Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.
Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.
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DOI：
10.1016/j.sbi.2013.04.003
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发表时间：
2013-06
影响因子：
6.8
通讯作者：
Owens RJ
中科院分区：
文献类型：
--
作者：
Aricescu AR;Owens RJ
哺乳动物细胞正迅速成为生产用于结构生物学应用的重组糖蛋白的首选系统。它们的使用使一整套新的目标，包括大，多结构域和高度糖基化的真核细胞表面受体及其超分子组装的结构研究。我们总结了在哺乳动物表达技术中取得的技术进步，并强调了使用这些方法获得的一些结构见解。展望未来，很明显，哺乳动物细胞表达将提供令人兴奋的和独特的机会，为蛋白质的结构研究的综合方法，特别是人类起源和医学相关的，通过弥合纯化状态和细胞环境之间的差距。
Mammalian cells are rapidly becoming the system of choice for the production of recombinant glycoproteins for structural biology applications. Their use has enabled the structural investigation of a whole new set of targets including large, multi-domain and highly glycosylated eukaryotic cell surface receptors and their supra-molecular assemblies. We summarize the technical advances that have been made in mammalian expression technology and highlight some of the structural insights that have been obtained using these methods. Looking forward, it is clear that mammalian cell expression will provide exciting and unique opportunities for an integrative approach to the structural study of proteins, especially of human origin and medically relevant, by bridging the gap between the purified state and the cellular context.