Steroid and β-adrenergic receptor modifications in target organs of broiler chickens fed with a diet containing β2-adrenergic agents
Steroid and β-adrenergic receptor modifications in target organs of broiler chickens fed with a diet containing β2-adrenergic agents
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DOI：
10.1016/j.fct.2008.02.025
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发表时间：
2008-06-01
影响因子：
4.3
通讯作者：
Re, G.
中科院分区：
文献类型：
--
作者：
Badino, P.;Odore, R.;Re, G.
非法使用β（2）-激动剂作为再分配剂以改善生产性能和屠体组成，可引起受影响动物的各种器官发生变化。本研究旨在探讨饲粮β受体激动剂对雄性肉鸡靶器官β-AR、AnR和GR的影响。将54只26日龄的雄性肉鸡（Ross 508）随机分为3个同质实验组，用含有安慰剂（组1，对照）、1 ppm的克伦特罗（组2）和1 ppm的西马特罗（组3）的标准饮食喂养21天。死后收集心脏、肺、胸腺和法氏囊的组织样品，然后将胞浆组分用于AnR（睾丸）和GR（脾脏、胸腺和法氏囊），并将膜组分用于通过结合试验测定β-AR（除睾丸外的所有组织）。饮食管理的β-肾上腺素能药物作为再分配剂诱导睾丸中的AnR浓度，在淋巴组织中的GR水平和雄鸡的不同靶器官中的β-AR浓度显著降低。目前的数据证实了在雌性鸡中观察到的结果，并表明在家禽中，肾上腺素能刺激对类固醇受体浓度的调节产生了与哺乳动物不同的影响。(C)2008爱思唯尔有限公司保留所有权利。
The illegal use of beta(2)-agonists as repartitioning agents to improve production performance and carcass composition can induce changes in various organs of exposed animals. The aim of the present study was to evaluate the effects induced by dietary beta-agonists on beta-AR, AnR and GR in male broiler target organs. Fifty-four male broiler chickens (Ross 508), 26 days old, were randomly divided into three homogeneous experimental groups and fed for 21 days with a standard diet containing placebo (group 1, control), 1 ppm of clenbuterol (group 2) and 1 ppm of cimaterol (group 3). Tissue samples of heart, lung, thymus and Bursa of Fabricius were collected post-mortem then cytosol fractions were used for AnR (testicles) and GR (spleen, thymus and Bursa of Fabricius), and membrane fractions for beta-AR (all tissues but testicles) determination by binding assays. The dietary administration of beta-adrenergic agents as repartitioning agents induced a significant decrease in AnR concentration in the testicle, in GR levels in the lymphoid tissues and in beta-AR concentrations of different target organs of male chickens. Present data confirm those observed in female chickens and suggest that in poultry the regulation exerted by adrenergic stimulation on steroid receptor concentrations produces different effects than in mammals. (C) 2008 Elsevier Ltd. All rights reserved.