Turnover time of Leydig cells and other interstitial cells in testes of adult rats.
Turnover time of Leydig cells and other interstitial cells in testes of adult rats.
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DOI：
10.3109/01485018908986831
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
K. Teerds;D. D. de Rooij-D.;F. Rommerts;I. van der Tweel;C. Wensing
中科院分区：
文献类型：
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作者：
K. Teerds;D. D. de Rooij-D.;F. Rommerts;I. van der Tweel;C. Wensing
本研究的目的是研究成年大鼠睾丸中 Leydig 细胞和其他间质细胞的更新情况。正常成年大鼠在9:00和21:00接受[3H]胸苷注射，持续2、5或8天。标记的间质细胞的百分比最初较低（0.8% +/- 0.2%），在治疗过程中逐渐增加至 1.4% +/- 0.3%。在[3H]胸苷治疗期间，标记的管周细胞的百分比相当高，并从1.4%+/-0.1%增加到3.5%+/-0.4%。剩余的间质细胞是增殖最活跃的细胞：在[3H]胸苷治疗期间，标记细胞的百分比从2.4%+/-0.2%增加到7.5%+/-0.8%。成年大鼠睾丸中的间质细胞、管周细胞和剩余的间质细胞发生增殖。通过线性回归分析和反正弦变换，获得了更换各种类型的间质细胞所需的时间间隔的估计。考虑到 95% 置信区间，间质细胞的更新时间范围为 142 至 2823 天。计算出管周细胞的周转时间为 85 至 257 天。
The aim of this study was to investigate the turnover of Leydig cells and other interstitial cells in the adult rat testis. Normal adult rats received injections of [3H]thymidine at 9:00 and 21:00 for 2, 5, or 8 days. The percentage of labeled Leydig cells, which was initially low (0.8% +/- 0.2%), gradually increased during treatment to 1.4% +/- 0.3%. The percentage of labeled peritubular cells was considerably higher and increased from 1.4% +/- 0.1% to 3.5% +/- 0.4% during [3H]thymidine treatment. The remaining interstitial cells were the most actively proliferating cells: the percentage of labeled cells increased from 2.4% +/- 0.2% to 7.5% +/- 0.8% during [3H]thymidine treatment. Leydig cells, peritubular cells, and the remaining interstitial cells in the adult rat testis undergo proliferation. By means of a linear regression analysis and an arcsin transformation, an estimation of the time interval needed to replace various types of interstitial cells was obtained. Taking into account the 95% confidence interval, the turnover time of Leydig cells ranged from 142 to 2823 days. The calculated turnover time for the peritubular cells was 85 to 257 days.