Reporter proteins for in vivo fluorescence without oxygen
Reporter proteins for in vivo fluorescence without oxygen
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DOI：
10.1038/nbt1293
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发表时间：
2007-04-01
影响因子：
46.9
通讯作者：
Jaeger, Karl-Erich
中科院分区：
文献类型：
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作者：
Drepper, Thomas;Eggert, Thorsten;Jaeger, Karl-Erich
荧光报告蛋白如绿色荧光蛋白是用于活体标本的体内真实的实时成像的有价值的非侵入性分子工具。然而，它们的使用通常限于有氧系统，因为它们的发色团的形成严格需要氧气。从枯草芽孢杆菌和恶臭假单胞菌的蓝光光感受器开始，它们含有光-氧-电压-感应结构域，我们设计了基于黄素单核苷酸的荧光蛋白，它们可以在有氧和厌氧生物系统中用作荧光报告基因。
Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real- time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue- light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light- oxygen- voltage - sensing domains, we engineered flavin mononucleotide - based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.