Multiple sclerosis normal-appearing white matter: pathology-imaging correlations.
Multiple sclerosis normal-appearing white matter: pathology-imaging correlations.
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DOI：
10.1002/ana.22521
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发表时间：
2011-11
影响因子：
11.2
通讯作者：
Fisher, Elizabeth
中科院分区：
文献类型：
--
作者：
Moll, Natalia M.;Rietsch, Anna M.;Thomas, Smitha;Ransohoff, Amy J.;Lee, Jar-Chi;Fox, Robert;Chang, Ansi;Ransohoff, Richard M.;Fisher, Elizabeth
确定多发性硬化（MS）患者大脑中正常白色物质（NAWM）磁化传递比（MTR）和弥散张量成像（DTI）参数细微异常的病理基础。通过快速尸检方案（包括原位MRI）获得脑组织。分析了四种类型的MRI定义的感兴趣区域（ROI）：（1）所有图像上异常的区域（“T2 T1 MTR病变”）;（2）MTR轻微异常的NAWM区域位于白色病变附近（“sa-WM关闭”）;（3）MTR轻微异常的NAWM区域位于远离病变的位置（“sa-WM远”）;以及（4）MTR正常的NAWM区域（“NAWM”）。每个感兴趣区的免疫组织化学分析包括髓鞘、轴突标记物、活化的小胶质细胞/巨噬细胞、星形胶质细胞、血浆蛋白和血管的免疫染色。分析了来自四个继发进行性MS脑的四十八个ROI。Sa-WM关闭ROI与显著更多的轴突sweep相关。与NAWM相比，在sa-WM Far、sa-WM Close和T2 T1 MTR病变中检测到更多的增大的MHCII（+）小胶质细胞和巨噬细胞。在所有ROI中，MTR和DTI测量与髓鞘密度、轴突面积和轴突计数中度相关。从分析中排除T2 T1 MTR病变显示，非病变WM中的MTR和DTI测量与活化的小胶质细胞相关，但与轴突或髓鞘完整性无关。NAWM中MRI异常的病理学基础根据与病灶WM病变的距离而变化。接近WM病变，轴突病理和小胶质细胞活化可以解释微妙的MRI变化。远离病变，小胶质细胞激活与邻近皮质病变可能是MRI异常的基础。
To determine the pathologic basis of subtle abnormalities in magnetization transfer ratio (MTR) and diffusion tensor imaging (DTI) parameters observed in normal-appearing white matter (NAWM) in multiple sclerosis (MS) brains. Brain tissues were obtained through a rapid post-mortem protocol that included in situ MRI. Four types of MRI-defined regions of interest (ROIs) were analyzed: (1) Regions that were abnormal on all images (“T2T1MTR lesions”); (2) NAWM regions with slightly-abnormal MTR located close to white matter lesions (“sa-WM Close”); (3) NAWM regions with slightly-abnormal MTR located far from lesions (“sa-WM Far”); and (4) NAWM regions with normal MTR (“NAWM”). Immunohistochemical analysis for each ROI comprised immunostaining for myelin, axonal markers, activated microglia/macrophages, astrocytes, plasma proteins and blood vessels. Forty-eight ROIs from four secondary progressive MS brains were analyzed. Sa-WM Close ROIs were associated with significantly more axonal swellings. There were more enlarged MHCII(+) microglia and macrophages detected in sa-WM Far, sa-WM Close, and T2T1MTR lesions than in NAWM. Across all ROIs, MTR and DTI measures were moderately correlated with myelin density, axonal area and axonal counts. Excluding T2T1MTR lesions from analysis revealed that MTR and DTI measures in non-lesional WM were correlated with activated microglia, but not with axonal or myelin integrity. The pathologic substrates for MRI abnormalities in NAWM vary based on distance from focal WM lesions. Close to WM lesions, axonal pathology and microglial activation may explain subtle MRI changes. Distant from lesions, microglial activation associated with proximity to cortical lesions might underlie MRI abnormalities.
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影响因子：
--
作者：
Bo, Lars;Geurts, Jeroen J. G.;Barkhof, Frederik
通讯作者：
Barkhof, Frederik
影响因子：
--
作者：
Moll, Natalia M.;Cossoy, Michael B.;Fisher, Elizabeth;Staugaitis, Susan M.;Tucky, Barbara H.;Rietsch, Anna M.;Chang, Ansi;Fox, Robert J.;Trapp, Bruce D.;Ransohoff, Richard M.
通讯作者：
Ransohoff, Richard M.
影响因子：
6
作者：
Ciccarelli, O;Werring, DJ;Thompson, AJ
通讯作者：
Thompson, AJ
影响因子：
14.5
作者：
Evangelou, N;Konz, D;Matthews, PM
通讯作者：
Matthews, PM
影响因子：
11.2
作者：
Filippi, M;Rocca, MA;Comi, G
通讯作者：
Comi, G