TRANSLATION RATES AND MISREADING CHARACTERISTICS OF RPSD MUTANTS IN ESCHERICHIA-COLI
TRANSLATION RATES AND MISREADING CHARACTERISTICS OF RPSD MUTANTS IN ESCHERICHIA-COLI
复制标题
DOI：
10.1007/bf00332630
复制
发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
KURLAND, CG
中科院分区：
文献类型：
--
作者：
ANDERSSON, DI;BOHMAN, K;KURLAND, CG
三个核糖体歧义（Ram）突变体，改变核糖体蛋白S4，在体内和体外的延伸率和翻译的误读进行了检查。与野生型相比，Ram突变体增加了体内无义密码子的误读，增加了2-50倍，这取决于无义密码子的性质、其位置和存在哪个rpsD等位基因。Ram核糖体在体外也显示出增加的错误频率。翻译的延伸率似乎没有显着改变，无论是在体内还是在体外，无论哪个rpsD等位基因的存在。显然，在Ram菌株中，翻译的准确性和整体速度之间没有关系。
Three ribosomal ambiguity (Ram) mutants, changed in ribosomal protein S4, were examined with respect to elongation rate and misreading of translation in vivo and in vitro. Ram mutants increase misreading of nonsense codons in vivo, compared to wild type, between 2-50 times depending on the nature of the nonsense codon, its position and which rpsD allele is present. Ram ribosomes also show an increased error frequency in vitro. The elongation rate of translation does not seem to be significantly changed, neither in vivo nor in vitro, irrespective of which rpsD allele is present. Apparently, there is no relationship between the accuracy and the overall speed of translation in Ram strains.